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©2007 Baishideng Publishing Group Inc.
World J Gastroenterol. Oct 21, 2007; 13(39): 5169-5179
Published online Oct 21, 2007. doi: 10.3748/wjg.v13.i39.5169
Published online Oct 21, 2007. doi: 10.3748/wjg.v13.i39.5169
Table 1 Strategies designed to inhibit HIV replication via RNA interference
Target gene | RNAi inducer (length) | Promoter | Vector | Cell type | Delivery methods | Inhib. of virus prod. (fold) | Reference |
Viral Gene | |||||||
LTR, Vif, Nef | siRNA (21 bp)/shRNA (19 bp)1 | T7 | Plasmid | Magi, PBLs | Transfection | > 20 | [47] |
Gag, Pol | siRNA (21 bp) | - | - | HOS.T4.CXCR4 | Transfection | > 10 | [48] |
Gag, LTR | siRNA (23 bp)/dsRNA (21nt)2 | - | - | U87-CD4+-CCR5+/CXCR4+, PBMC | Transfection | 4 | [51] |
Gag, Env | dsRNA (441-531nt)3 | - | - | COS, Hela-CD4+, PBMC, ACH2 | Transfection | 70 | [52] |
Tat + Rev | siRNA (21 bp) | - | - | 293T, Jurkat, PBMC | Transfection | > 15 | [54] |
Rev (Tat) | siRNA (21 bp)5 | Dual U6 | Plasmid | 293/EcR | Transfection | 10000 | [56] |
Nef | dsRNA (556nt)3 | - | - | MT4-T, U937 | Transfection | 2.5 | [57] |
Env | siRNA (20 bp)/shRNA (20 bp)4 | U6 | Plasmid, Lentivirus | COS, MT-4 | Transfection /Transduction | > 10 | [53] |
Nef | shRNA (21 bp) | H1 | Retrovirus | SupT1 | Transduction | > 10 | [58] |
Gag, Pol, Int, Vpu | shRNA (21 bp) | H1 | Lentivirus | 293T, Magi, GHOST hi5, CEM-A, Molt-4, PBMC | Transduction | > 20 | [61] |
Cellular gene | |||||||
Tsg101 | siRNA (21 bp) | - | - | 293T | Transfection | 10-20 | [63] |
LEDGF/p75 | siRNA (21 bp) | - | - | Hela | Transfection | NR | [64] |
P-TEFb (CDK9/CyclinT1) | siRNA (21 bp) | - | - | Hela, Magi | Transfection | 3-5 | [65] |
hRIP | siRNA (21 bp) | - | - | Hela, Jurkat, Macrophages | Transfection | -100 | [66] |
Emerin | siRNA (21 bp) | - | - | Hela, Macrophages | Transfection | > 10 | [67] |
LEDGF/p75, HRP2 | siRNA (21 bp) | - | - | Hela-P4 | Transfection | 2-3 | [68] |
CXCR4 | siRNA (21 bp) | - | - | HOS-CD4+, HOS-CD4+-CXCR4+/CCR5+ | Transfection | 3-5 | [70] |
Importin 7 | siRNA (21 bp) | - | - | Hela, Macrophages | Transfection | -10 | [69] |
CXCR4+ CD4, | |||||||
CXCR4+ CCR5 | shRNA (19/21 bp)6 | - | - | Magi-CXCR4/CCR5, PBMC | Transfection | > 15 | [71] |
CCR5 | shRNA (19 bp) | U6 | Lentivirus | Magi-CCR5, PBLs | Transduction | 3-7 | [34] |
Combination of viral and cellular genes | |||||||
Gag, CD4 | siRNA (21 bp) | - | - | Magi-CCR5, Hela-CD4 | Transfection | 4-25 | [50] |
Tat, RT, NF-κB (p65) | siRNA (21 bp) | - | - | Magi, Jurkat | Transfection | 5-500 | [74] |
Table 2 Strategies designed to inhibit HCV replication via RNA interference
Target gene | RNAi inducer (length) | Promoter | Vector | Model | Delivery methods | Inhib. of virus prod. (fold) | Reference |
In vitro studies | |||||||
Viral gene | |||||||
5’-UTR | siRNA (21 bp) | - | - | 5-2 cells (Huh-7) | Transfection | -6 | [79] |
siRNA (21 bp)/shRNA(19 bp)1 | U6 | Plasmid | 293T, Huh 7 | Transfection | > 10 | [78] | |
NS4B | siRNA (23 bp) | - | - | Huh-7.5 | Transfection | -80 | [80] |
NS3, NS4B, NS5A, NS5B | siRNA (21 bp) | - | - | S1179I (Huh-7) | Transfection | -23 | [81] |
IRES, NS3, NS5B | siRNA (23bp)/shRNA (21 bp)2 | Dual H1 | Plasmid | Huh-7 | Transfection | > 9 | [82] |
5’-UTR, C, NS4B, NS5A, NS5B | esiRNA (15-40 bp)3/shRNA (19bp) | H1 | Mo-MuLV | Huh-7 | Transfection / Transduction | -100 | [88] |
5’-UTR, C, NS3, NS5B | siRNA (21 bp)/shRNA (19 bp)4 | U6 | Plasmid/Lentivirus | Huh-7 | Transfection / Transduction | -7 | [83] |
Cellular gene | |||||||
Lα, PTB, eIF2Bγ, hVAP33 | shRNA (19 bp) | U6 | Plasmid/Adenovirus | Huh-7 | Transfection / Transduction | -13 | [91] |
Cyp-A,B,C | shRNA (NR) | U6 | Plasmid/Retrovirus | Huh-7 | Transfection / Transduction | -10 | [92] |
Combination of viral and cellular genes | |||||||
5’-UTR, 3’-UTR, PSMA7, HuR | shRNA (19-21 bp) | U6 | Plasmid/Retrovirus | Huh-7 | Transfection / Transduction | > 2 | [89] |
CD81, IRES, NS5B | shRNA (19-21 bp) | H1 | Lentivirus | Huh-7 | Transduction | > 32 | [90] |
In vivo studies | |||||||
NS5B | siRNA (23 bp) | - | - | Mice | Hydrodynamic transfection | 3 | [84] |
IRES | shRNA (19-25 bp)5 | - | - | Mice | Hydrodynamic transfection | -50 | [85] |
Table 3 Strategies designed to inhibit HBV replication via RNA interference
Target gene | RNAi inducer (length) | Promoter | Vector | Model | Delivery methods | Inhib. of virus prod. (fold) | Reference |
In vitro studies | |||||||
C | siRNA (21 bp) | - | - | Huh-7, HepG2 | Transfection | -4-5 | [95] |
siRNA (19 bp) | - | - | HepAD38, HepAD79 | Transfection | -50 | [96] | |
C, X | shRNA (19 bp) | hH1 | Plasmid | Huh-7, HepG2.2.15 | Transfection | 2-20 | [97] |
C, S, P, X, DR | shRNA (21-24 bp) | mU6 | Plasmid | HepG2.2.15 | Transfection | -2 | [98] |
shRNA (21 bp) | hU6 | Plasmid | HepG2 | Transfection | > 30 | [105] | |
S | shRNA (19 bp) | hH1 | PFV, AAV | 293T.HBs, HepG2.2.15 | Transduction | 4-9 | [99] |
In vivo studies | |||||||
C, S, P, X | shRNA (25 bp) | hU6 | Plasmid | Immunocompetent C57BL/6J mice, Immunocompromised NOD/SCID mice | Hydrodynamic transfection1 | 3-12 | [100] |
C, S | siRNA (21 bp) | - | - | Male NMRI mice | High-volume injection via tail vein1 | -4 | [101] |
S | shRNA (19 bp) | hH1, hU6 | Plasmid | BALB/c mice, HBsAg-transgenic FVB/N mice | Hydrodynamic transfection2 | -9 | [102] |
P, S, X | shRNA (20 bp) | hH1 | Plasmid | C57BL/6 HBV-transgenic mice | Hydrodynamic transfection1 | 19-99 | [103] |
P, S, X | shRNA (NR) | mU6 | Adenovirus | HBV-transgenic mice | Hydrodynamic transfection | > 9 | [104] |
Table 4 Strategies designed to inhibit SARS-CoV replication via RNA interference
Target gene | RNAi inducer (length) | Promoter | Vector | Model | Delivery methods | Inhib. of virus prod. (fold) | Reference |
In vitro studies Viral gene | |||||||
Leader, TRS, 3’-UTR, S | siRNA (21 bp) | - | - | Vero E6 | Transfection | 9 | [110] |
N | shRNA (20 bp) | U6 | Plasmid | 293 | Transfection | NR | [112] |
E, M, N | siRNA (21 bp) | - | - | Vero E6 | Transfection | > 4 | [113] |
P | shRNA (19 bp) | H1 | Plasmid | Vero | Transfection | > 100 | [114] |
S | shRNA (22 bp) | U6 | Plasmid | Vero E6, 293T | Transfection | -6 | [115] |
Rep | siRNA (21 bp) | - | - | FRhk-4 | Transfection | > 12 | [118] |
Cellular gene | |||||||
CHC | siRNA (25 bp) | - | - | HepG2, COS7 | Transfection | -1 | [116] |
In vivo studies | |||||||
S, NSP12 | siRNA (21 bp) | - | - | BALB/C mouse, Rhesus macaque (Macaca mulatta) | i.t.1 and i.n.2 administration | 3 | [119] |
- Citation: Ma Y, Chan CY, He ML. RNA interference and antiviral therapy. World J Gastroenterol 2007; 13(39): 5169-5179
- URL: https://www.wjgnet.com/1007-9327/full/v13/i39/5169.htm
- DOI: https://dx.doi.org/10.3748/wjg.v13.i39.5169