Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis

Table 1 Comparison of different indexes level in blood [M (Q_R)]

		Sham operation group	Model group	Baicalin treated group	Octreotide treated group
Endotoxin (EU/mL)	3 h	0.016 (0.005)	0.053 (0.029)	0.027 (0.005)	0.033 (0.006)
	6 h	0.016 (0.010)	0.059 (0.037)	0.039 (0.019)	0.031 (0.010)
	12 h	0.014 (0.015)	0.060 (0.022)	0.034 (0.015)	0.042 (0.014)
BUN (mmol/L)	3 h	5.310 (0.940)	12.050 (4.030)	10.530 (3.625)	9.850 (3.020)
	6 h	5.500 (2.200)	17.390 (3.850)	12.220 (4.530)	13.930 (5.500)
	12 h	4.860 (1.590)	22.270 (11.375)	13.720 (4.380)	13.520 (9.810)
NO (μmol/L)	3 h	7.500 (5.000)	65.000 (7.50)	57.500 (22.50)	52.500 (15.00)
	6 h	7.500 (5.000)	62.500 (38.75)	47.500 (37.50)	57.500 (15.00)
	12 h		74.100 (26.15)	57.500 (27.50)	45.000 (12.50)
TNF-α (pg/mL)	3 h	3.900 (3.200)	41.440 (37.72)	44.930 (45.84)	39.300 (30.60)
	6 h	4.000 (1.700)	92.150 (23.12)	65.100 (27.51)	47.600 (16.50)
	12 h	5.3000 (3.000)	65.020 (26.81)	47.650 (25.52)	54.500 (41.40)
IL-6 (pg/mL)	3 h	1.846 (0.346)	5.437 (1.025)	3.031 (0.870)	2.646 (1.373)
	6 h	1.743 (0.838)	6.817 (0.810)	2.882 (1.392)	3.076 (1.205)
	12 h	2.036 (0.818)	5.356 (0.747)	2.832 (0.597)	2.462 (1.353)
ET-1 (pg/mL)	3 h	15.293 (4.231)	24.745 (1.011)	19.635 (6.065)	16.827 (3.775)
	6 h	16.275 (3.180)	25.625 (7.973)	16.226 (3.174)	14.855 (5.747)
	12 h	14.173 (2.556)	24.725 (3.759)	18.625 (5.780)	15.185 (1.761)

Table 2 Comparison of serum CREA content (mean ± SD, μmol/L)

Groups	3 h	6 h	12 h
Sham operation group	17.867 ± 2.890	21.467 ± 3.044	19.733 ± 3.150
Model group	38.400 ± 11.344	45.154 ± 17.435	41.500 ± 12.122
Baicalin-treated group	37.615 ± 9.483	39.867 ± 13.648	50.733 ± 29.310
Octreotide-treated group	29.200 ± 5.710	33.533 ± 10.106	33.933 ± 9.145

Table 3 Pathological score standard of kidney

Grade	Observation indexes
I	No cellular proliferation or fibrosis in renal glomerulus; no capillary congestion or microthrombus; swelling and blurry boundary of renal tubular epithelial cell; stegnosis or atresia of lumens; protein cast and renal interstitial edema
II	Glomerular capillary congestion, scattered necrosis in renal tubular epithelial cell, interstitial edema and inflammatory cell infiltration
III	II + lamellar necrosis of renal tubular epithelial cell

Table 4 Comparision of pathological score of kidney in all groups [M (Q_R)]

Groups	3 h	6 h	12 h
Sham operation group	0.00 (0.00)	0.00 (0.00)	0.00 (0.00)
Model group	2.00 (1.00)	2.00 (1.00)	2.00 (1.25)
Baicalin treated group	1.00 (1.00)	1.00 (1.00)	2.00 (1.00)
Octreotide-treated group	1.00 (1.00)	1.00 (1.00)	1.00 (1.00)

Table 5 Expression of Bax protein in kidney

Groups		n	Pathologic grade
			-	+	++	+++
Sham operation group	(3 h)	15	15
	(6 h)	15	15
	(12 h)	15	15
Model group	(3 h)	15	7	4	4
	(6 h)	13	10	1	2
	(12 h)	10	7	1	1	1
Baicalin treated group	(3 h)	15	11	3	1
	(6 h)	15	10	4	1
	(12 h)	15	12	3
Octreotide treated group	(3 h)	15	12	3
	(6 h)	15	11	2	2
	(12 h)	15	10	4	1

Table 6 Comparision of Bax protein in kidney [M (Q_R)] grade score

Groups	3 h	6 h	12 h
Sham operation group	0.00 (0.00)	0.00 (0.00)	0.00 (0.00)
Model group	1.00 (2.00)	0.00 (0.50)	0.00 (1.25)
Baicalin-treated group	0.00 (0.50)	0.00 (1.00)	0.00 (0.00)
Octreotide-treated group	0.00 (0.00)	0.00 (1.00)	0.00 (1.00)

Table 7 Expression of Bcl-2 protein in kidney

Groups		n	Pathologic grade
			-	+	++	+++
Sham operation group	(3 h)	15	15
	(6 h)	15	15
	(12 h)	15	15
Model group	(3 h)	15	10	1	2	2
	(6 h)	13	4		1	8
	(12 h)	10	7	1	2
Baicalin-treated group	(3 h)	15	14	1
	(6 h)	15	15
	(12 h)	15	11	4
Octreotide-treated group	(3 h)	15	13	2
	(6 h)	15	15
	(12 h)	15	15

Table 8 Comparison of Bcl-2 protein in kidney [M (Q_R)] grade score

Groups	3 h	6 h	12 h
Sham operation group	0.00 (0.00)	0.00 (0.00)	0.00 (0.00)
Model group	0.00 (2.00)	3.00 (3.00)	0.00 (1.25)
Baicalin-treated group	0.00 (0.00)	0.00 (0.00)	0.00 (1.00)
Octreotide-treated group	0.00 (0.00)	0.00 (0.00)	0.00 (0.00)

Table 9 Apoptotic index of kidney [M (Q_R)] (%)

Groups	3 h	6 h	12 h
Sham operation group	0.00 (0.00)	0.00 (0.00)	0.00 (0.00)
Model group	0.00 (0.00)	0.00 (0.00)	0.00 (0.00)
Baicalin-treated group	0.00 (0.01)	0.00 (0.02)	0.00 (0.00)
Octreotide-treated group	0.00 (0.00)	0.00 (0.04)	0.00 (0.00)

Table 10 Comparison of serum PLA2 content (mean ± SD, U/mL)

Groups	3 h	6 h	12 h
Sham operation group	14.62 ± 3.02	17.49 ± 3.82	19.02 ± 5.07
Model group	76.10 ± 16.70	101.46 ± 14.67	105.33 ± 18.10
Baicalin-treated group	56.25 ± 22.43	67.91 ± 20.61	66.86 ± 22.10
Octreotide-treated group	74.37 ± 19.94	63.13 ± 26.31	53.63 ± 12.28