Protective effect and mechanism of stronger neo-minophagen C against fulminant hepatic failure

Table 1 Levels of NO, TNF-α, ET-1, IL-6, ALT, ALB, and TBIL in control, model and treatment groups(mean±SD)

Groups	Mice (n)	NO (μmol/L)	TNF-α (ng/mL)	ET-1 (pg/mL)	IL-6 (pg/mL)	ALT (u/L)	ALB (g/L)	TBIL (u/L)
Control group	6	45.1 ± 14.01	0.77 ± 0.08	50.8 ± 7.58	57.07 ± 12.67	67.17 ± 12.62	32.18 ± 4.16	9.2 ± 8.75
Model group	6	725.94 ± 156.94	3.75 ± 0.50	309.41 ± 38.45	413.56 ± 69.02	1406.33 ± 47.42	21.13 ± 3.66	70.03 ± 17.22
Treatment group	6	199.42 ± 85.98	1.34 ± 0.31	129.11 ± 17.97	155.22 ± 59.37	799.33 ± 171.19	20.03 ± 3.01	8.87 ± 0.85
140 mL group	6	215.01 ± 28.72	1.35 ± 0.17	183.07 ± 75.93	149.81 ± 44.69	25.96 ± 6.35	26.10 ± 1.97	59 ± 29.31
200 mL group	6	87.88 ± 12.46	1.34 ± 0.31	157.41 ± 35.64	133.89 ± 30.48	1169 ± 149.92	20.9 ± 1.28	27.33 ± 2.94
280 mL group	6	184.54 ± 19.84	1.27 ± 0.27	216.15 ± 24.47	179.97 ± 65.09	24.33 ± 8.02	21.37 ± 5.44	51.5 ± 14.94

F_(4,29) = 2.70.

Table 2 Hepatocyte apoptosis index and number of cells expressing Cyt-C and caspase-3 (mean ± SD, number/HP)

Groups	Number	Apoptosis index	Cyt-C	Caspase-3
Control group	5	0	1.67 ± 0.90	3.87 ± 2.42
Model group	5	33.2 ± 4.37	59.47 ± 3.79	85.6 ± 6.25
After therapy 6 h	5	32.3 ± 4.7	58.47 ± 3.83	84.00 ± 5.54
After therapy 1 d	5	26.6 ± 4.67b	46.33 ± 5.51b	72.3 ± 5.21b
After therapy 3 d	5	19.9 ± 3.54b	31.53 ± 2.83b	56.13 ± 6.87b
After therapy 5 d	5	11.1 ± 2.77b	25.47 ± 3.94b	34.1 ± 4.87b
After therapy 7 d	5	5 ± 2.83b	15.00 ± 4.11b	17 ± 4.5b

^bP < 0.01 vs the model group. Apoptosis index: t_6h = 0.56, t_1d = 4.10, t_3d = 8.26, t_5d = 13.73, t_7d = 17.52. Cyt-c: t_6h = 0.72, t_1d = 9.45, t_3d = 20.1, t_5d = 24.46, t_7d = 31.99. Caspase-3: t_6h = 1.09, t_1d = 12.2, t_3d = 26.9, t_5d = 47.2, t_7d = 62.9.