Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters

Table 1 Risk categories in GISTs

	Size	Mitotic count
Very low risk	< 2 cm	< 5/50 HPF
Low risk	2-5 cm	< 5/50 HPF
Intermediate risk	< 5 cm	6-10/50 HPF
	5-10 cm	< 5/50 HPF
High risk	> 5 cm	> 5/50 HPF
	> 10 cm	Any mitotic rate
	Any size	> 10/50 HPF

Table 2 Clinicopathological features of 25 patients with GISTs

Variables	Cases n (%)
Sex
Male	16 (64)
Female	9 (36)
Site
Stomach	11 (44)
Small intestine	11 (44)
Large intestine	3 (12)
Tumor size (cm)
< 2 cm	0 (0)
2-5 cm	4 (16)
5-10 cm	16 (64)
> 10 cm	5 (20)
Mitosis
< 5	11 (44)
≥ 5	14 (56)
Diagnosis
Very low risk	0 (0)
Low risk	3 (12)
Intermediate risk	6 (24)
High risk	16 (64)

Table 3 Immunostaining results of 25 cases of GISTs

Site of origin	c-kit	COX-2	CD34	SMA +	SMA +	SMA -	SMA -
	+	+	+	S-100 +	S-100 -	S-100 +	S-100 -
Stomach (n = 11)
Low risk	2	1	2	-	-	1	1
Intermediate risk	4	4	3	-	-	2	2
High risk	5	3	4	2	-	1	2
Small intestine
(n = 11)
Low risk	1	1	1	-	1	-	-
Intermediate risk	2	1	2	1	-	1	-
High risk	7	6	6	2	2	4	-
Large intestine
(n = 3)
High risk	2	3	3	2	-	-	1
Total n (%)	23 (92)	19 (76)	21 (84)	7 (28)	3 (12)	9 (36)	6 (24)

Table 4 The results of immunohistochemical staining for p53

p53 index	0	1	2	3	4
Low risk	1	1	1	0	0
Intermediate risk	0	2	1	1	2
High risk	6	6	2	2	0
Total	7	9	4	3	2