A novel sampling method for the investigation of gut microbiota

Table 1 Mean concentration and prevalence of microorganisms at each site in the LDT

Microorganisms					Colon
	Ileum		Cecum		Ascending		Transverse		Descending		Sigmoid		Rectum
	MC	%	MC	%	MC	%	MC	%	MC	%	MC	%	MC	%
Bacillus sp	1	9.5	2	4.2	5	4.2	3	4.2			1	4.3	3	5
Bacteroide sp (pig.)	5	4.8									5	4.3	1	5
Bacteroides sp (npg)	3	47.6	5	29.2	4	37.5	5	12.5	5	12.5	5	47.8	5	65
Bacteroides sp	2	4.8					2	4.2					3	5
Bifidobacterium sp	2	9.5			3	4.2	4	4.2	4	8.3	4	8.7	3	5
Candida sp	3.5	9.5	2	20.8	3	20.8	2	29.2	4	16.7	3	34.8	3	35
Clostridium rammosum	1	4.8							3	4.2
Clostridium sp (gel -)	4	66.7	4	45.8	5	50	4.5	58.3	5	54.2	5	69.6	7	60
Clostridium sp (gel+)			2	4.2	4	4.2							3	5
Clostridium sp	1	4.8	4	45.8	7	4.2							5	5
Corynebacterium sp	3	57.1	3	54.2	3.5	50	4	41.7	4	45.8	5	60.9	5	65
E.coli	5	47.6	5	37.5	5	45.8	4.5	50	5	37.5	5	69.6	7	80
Enterobacter cloacae			4	4.2	1	4.2
Enterobacter sp	4	28.6	4	20.8	7	37.5	4	29.2	5	45.8	5	52.2	7	35
Enterococcus faecalis	4.5	9.5	3	4.2	4	4.2	3	4.2	1	4.2	4	8.7
Enterococcus faecium							5	4.2
Enterococcus sp	4	38.1	2.5	33.3	3.5	33.3	3	58.3	5	58.3	5	34.8	5	60
Eubacterium lentum	4	4.8
Eubacterium sp	3.5	9.5
Fusobacterium fusiformes	3	4.8
Fusobacterium sp	3.5	19	2.5	25	3	20.8	4	16.7	4	8.3	3	21.7	4	55
Klebsiella pneumoniae									5	4.2
Klebsiella sp	5	76.2	4	54.2	6	62.5	5	54.2	5	41.7	7	69.6	7	65
Lactobacillus acidophillus	4	4.8	3	4.2
Lactobacillus sp	4	33.3	2	29.2	2.5	33.3	3	37.5	3	25	2	43.5	4	70
Leptotrichia sp					1	4.2					1	4.3
Peptococcus anaerobius	2	4.8	4	4.2	2	4.2					1	4.3
Peptococcus assachalyticus	2	4.8
Peptococcus sp	2	28.6	2.5	33.3	3	25	3	33.3	3	25	3	56.5	3	35
Peptostreptococcus sp	3	9.5			2	8.3			1	4.2	5	13
Propionibacterium sp	3	52.4	3	20.8	4	33.3	3	50	3	29.2	5	26.1	5	30
Proteus	3.5	19	4	12.5	5	33.3	5	37.5	5	25	7	34.8	7	30
Pseudomonas sp	3	9.5					1	4.2
Rodothorula sp	4	14.3	2	20.8	1	4.2	1	12.5	3	16.7	1	4.3	3.5	20
Selenomonas sp													2	5
Staphylococcus sp	5	4.8	2	4.2
Staphylococcus sp (coag -)	2	33.3	2	20.8	1.5	8.3	2	20.8	2	8.3	3	21.7	3	45
Staphylococcus sp (coag +)													3	5
Streptococcus sp (coag - )
Streptococcus (gama hem)									2	8.3
Streptococcus sp (alfa hemolítico grupo Viridans)	4	14.3	4	16.7	2	8.3	3.5	8.3			2	13	3	5
Veillonella sp (Gel - )							3	8.3
Veillonella sp	4	90.5	2.5	83.3	4	70.8	4	62.5	5	75	5	95.7	5	90

MC = mean concentration (CFU/Log₁₀); % = prevalence.

Table 2 Statistically predominating genera at different sites in the LDT

Microbiota	LDT site
Klebisiella sp	Ileuma
Enterobacter sp	Sigmoida
E.coli, Enterococcus sp	Rectuma

sp = species; LDT: lower digestive tract;

^aP < 0.05 vs each of two sites.