Overexpression of the cholesterol-binding protein MLN64 induces liver damage in the mouse

Table 1 Primers for gene expression analysis in MLN64-overexpressing mouse livers

Gene	Primers	Fragment size
bax	TATTGGTGAGTCGGATTGC (S)	230 bp
	TGGACGGTCAGTGTCTGG (AS)
mdm2	CCAACATGTCTGTGTCTACCG (S)	216 bp
	ACAATGTGCTGCTGCTTCTC (AS)
mln64	TCGACATCTTTGTTCTGGCT (S)	148 bp
	GAGCAACTCAGAAAGGATGAC (AS)
18 S	GTAACCCGTTGAACCCCATT (S)	151 bp
	CCATCCAATCGGTAGTAGCG (AS)

S: sense; AS: antisense.

Table 2 Effects of MLN64 recombinant adenoviral infection on serum alanine aminotransferase and alkaline phosphatase (mean ± SE)

Group	ALT U/L	AP U/L
No virus	14.00 ± 7.04	55.25 ± 6.44
Ad.E1Δ	39.67 ± 4.91	76.33 ± 4.42
Ad.MLN64	3780 ± 1010a	209.50 ± 20.17a

Measurements were performed in four mice in each experimental group.

^aP < 0.05 vs Ad. E1Δ-infected mice.

Table 3 Effects of MLN64 recombinant adenoviral infection on hepatic cholesterol levels, biliary bile flow, and lipid secretion (mean ± SE)

		Hepatic cholesterol			Biliary lipid secretion
		Unesterified	Ester	Bile flow	Cholesterol	Bile salts	Phospholipids
Group	Liver weight	(mg/g liver weight)	(mg/g liver weight)	(μL/min/100 g body weight)	(nmol/min/100 g body weight)	(nmol/min/100 g body weight)	(nmol/min/100 g body weight)
Ad.E1Δ	1.32 ± 0.04 (n = 11)	1.97 ± 0.08	0.20 ± 0.08	9.43 ± 0.76 (n = 8)	2.75 ± 0.33	254.1 ± 34.2	84.25 ± 5.5
Ad.MLN64	1.14 ± 0.04 (n = 16)a	2.38 ± 0.08a	0.21 ± 0.07	4.14 ± 0.61a	1.38 ± 0.19a	308.5 ± 45.4	54.64 ± 13.5

Measurements were performed in six mice in each experimental group, except for liver weight and bile flow as indicated.

^aP < 0.05 vs Ad.E1Δ-infected mice.