Leptin treatment ameliorates acute lung injury in rats with cerulein-induced acute pancreatitis

Table 1 Biochemical markers in the blood, and pancreatic and lung tissues at 24 h mean ± SE

Groups	Amylase(U/L)	Lipase(U/L)	TNF-α(pg/mL)	IL-1b(pg/mL)	MIP(pg/mL)	sICAM(pg/mL)	Pancreas MPO(U/gr)	Lung MPO(U/gr)	Lung NOx(nmol/mg protein)
Sham Group	44.2 ± 5.8	17.2 ± 1.0	9.5 ± 0.7	6.4 ± 0.8	5.7 ± 8.5	11132 ± 626	0.17 ± 0.02	2.5 ± 0.6	8.1 ± 1.1
AP Group	1913 ± 205a	47.1 ± 9.0	114.6 ± 16.5a	107.9 ± 5.1a	128.3 ±5.1a	27828 ± 1483a	25.0 ± 11.1a	95.8 ± 17.0a	26.6 ± 0.8a
Leptin Group	1419 ± 87	39.4 ± 36.3	67.2 ± 7.0	67.2 ± 7.0	45.0 ± 10.2	21042 ± 1676	4.1 ± 1.6	38.2 ± 6.3	19.3 ± 0.7

^aP < 0.05 vs the leptin group.

Table 2 Biochemical markers in the blood, and pancreatic and lung tissues at 48h mean ± SE

Group	Amylase(U/L)	Lipase(U/L)	TNF-α(pg/mL)	IL-1b(pg/mL)	MIP(pg/mL)	sICAM(pg/mL)	Pancreas MPO(U/gr)	Lung MPO(U/gr)	Lung NOx(nmol/mg protein)
Sham group	37.3 ± 14.3	15.3 ± 2.1	5.5 ± 0.8	8.6 ± 3.7	8.5 ±3.8	14216 ± 1148	0.51 ± 0.29	6.2 ± 2.3	6.1 ± 0.8
AP group	1078 ± 77	44.2 ± 4.5	91.2 ± 2.2a	94.8 ± 7.5a	78.1 ± 9.7a	28416 ± 1321a	11.4 ± 2.5	97.6 ± 15.1a	21.9 ± 0.7
Leptin group	1233 ± 128	36.3 ± 1.7	53.6 ± 3.8	37.0 ± 6.1	28.9 ± 2.3	16712 ± 1054	5.0 ± 1.7	29.7 ± 2.9	15.3 ± 0.8

^aP < 0.05 vs the leptin group.

Table 3 Histological scores of pancreatic injury mean ± SE

Group	Edema		Inflammation		Vacuolization		Necrosis
	24 h	48 h	24 h	48 h	24 h	48 h	24 h	48 h
Sham group	0	0	0	0	0	0	0	0
AP group	2.57 ± 0.20a	2.37 ± 0.18a	1.28 ± 0.28	1.75 ± 0.46a	2.28 ± 0.35a	1.50 ± 0.16a	0.71 ± 0.18	1.00 ± 0.00a
Leptin group	1.14 ± 0.14	1.12 ± 0.12	0.57 ± 0.20	0.50 ± 0.18	0.42 ± 0.20	0.50 ± 0.18	0.28 ± 0.18	0.50 ± 0.14

^aP < 0.05 vs the leptin group.

Table 5 Comparison of pancreatic and pulmonary CD40 staining levels in different study groups

Group	Pancreas		Lung
	24 h	48h	24 h	48 h
Sham group	0	0	0	0
AP group	2.6 ± 0.8a	3.4 ± 0.4a	2.6 ± 0.8a	3.1 ± 0.6a
Leptin group	1.5 ± 0.6	2.1 ± 0.3	1.5 ± 0.6	1.9 ± 0.5

^aP < 0.05 vs the leptin group.

Table 4 Histological scores of pulmonary injury mean ± SE

Group	Alveolar edema		Alveolar distention		PMNC infiltration		Alveolar wall thickening
	24 h	48 h	24 h	48 h	24 h	48 h	24 h	48 h
Sham group	0	0	0	0	0	0	0	0
AP group	2.28 ± 0.35a	1.62 ± 0.18a	1.42 ± 0.36	1.25 ± 0.16	2.71 ± 0.18a	2.21 ± 0.12a	0.85 ± 0.40	2.00 ± 0.18a
Leptin group	1.28 ± 0.18	0.37 ± 0.18	1.25 ± 0.16	0.62 ± 0.51	0.57 ± 0.29	0.75 ± 0.16	0.42 ± 0.29	0.87 ± 0.22

^aP < 0.05 vs the leptin group.