Detection of let-7a microRNA by real-time PCR in gastric carcinoma

Table 1 Oligonucleotides used in this study

let-7aPRT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGAT ACGACAACTA

let-7aPf: GCCGCTGAGGTAGTAGGTTGTA

let-7aPr: GTGCAGGGTCCGAGGT

let-7aT: (6-FAM) TGGATACGACAACTATAC (MGB)

let-7aSeq: 6-UGAGGUAGUAGGUUGUAUAGUU-27 (MIMAT0000062)

Table 2 Expression of let-7a miRNA in gastric carcinoma tissue

Sample No.	T (copies/μg)			N (copies/μg)			T/N
	let-7a	β-actin	let-7a/β-actin	let-7a	β-actin	let-7a/β-actin
50901	7.37 × 104	1.05 × 105	0.7	2.15 × 105	1.43 × 105	1.5	0.47
51005	1.15 × 104	3.83 × 104	0.3	4.35 × 104	2.90 × 104	1.5	0.20
51006	1.03 × 105	5.15 × 105	0.2	8.57 × 105	4.76 × 105	1.8	0.11
51208	6.30 × 105	1.26 × 106	0.5	1.98 × 106	1.65 × 106	1.2	0.42
06031	1.03 × 103	8.58 × 102	1.2	2.63 × 103	1.05 × 103	2.5	0.48
60412	1.35 × 105	2.70 × 105	0.5	5.88 × 105	2.45 × 105	2.4	0.21
60418	6.53 × 105	1.09 × 106	0.6	2.74 × 106	1.37 × 106	2.0	0.30
60519	6.31 × 104	2.10 × 105	0.3	4.94 × 105	2.35 × 105	2.1	0.14
60524	3.05 × 105	7.63 × 105	0.4	6.22 × 105	6.91 × 105	0.9	0.44
60625	1.53 × 105	5.10 × 105	0.3	5.78 × 105	4.82 × 105	1.2	0.25
60726	8.24 × 104	1.03 × 105	0.8	2.55 × 105	9.46 × 104	2.7	0.30
60729	5.61 × 106	5.61 × 107	0.1	3.49 × 107	4.36 × 107	0.8	0.13
60731	4.35 × 104	8.70 × 104	0.5	1.32 × 105	9.41 × 104	1.4	0.36
60832	7.28 × 105	3.64 × 106	0.2	3.78 × 106	3.15 × 106	1.2	0.17

T: Gastric carcinoma tissue; N: Normal tissue; T/N: Difference in let-7a expression between tumor and normal tissue.