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©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Jan 14, 2007; 13(2): 257-263
Published online Jan 14, 2007. doi: 10.3748/wjg.v13.i2.257
Published online Jan 14, 2007. doi: 10.3748/wjg.v13.i2.257
Table 1 Sequences for quantitative RT-PCR and nested-PCR primers and flourescent probes
| Forward primer | Reverse primer | Length (bp) | |
| TaqMan primer CK-19 | 5'-GAAGGCCTGAAGGAAGAGCTG-3' | 5'-CCTCCCACTTGGCCCCT-3' | 80 |
| TaqMan primer beta-actin | 5'-TCCAGAGGCGCTCTTCCA-3' | 5'-CGCACTTCATGATCGAGTTGA-3' | 86 |
| Vector primer CK-19 | 5'-AACTCCAGGATTGTCCTGCAG-3' | 5'-TCCCGGTTCAATTCTTCAGTC-3' | 401 |
| Vector primer beta-actin | 5'-GCACCACTGGCATTGTCATG-3' | 5'-CCACACGGAGTACTTGCGC-3' | 581 |
| Nested-PCR primer CK-19 | 5'-AACTCCAGGATTGTCCTGCAG-3' | 5'-TCCCGGTTCAATTCTTCAGTC-3' | 401 |
| Nested-PCR primer CK-19 | 5'-GAAGGCCTGAAGGAAGAGCTG-3' | 5'-CCTCCCACTTGGCCCCT-3' | 80 |
| Probe CK-19 | 5'-CCTACCTGAAGAAGAACCATGAGGAGGAAATCAGTA-3' | ||
| Probe beta-actin | 5’-CCTCCTTCCTGGGCATGGAGTCCTG-3’ |
- Citation: Hoffmann K, Kerner C, Wilfert W, Mueller M, Thiery J, Hauss J, Witzigmann H. Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients. World J Gastroenterol 2007; 13(2): 257-263
- URL: https://www.wjgnet.com/1007-9327/full/v13/i2/257.htm
- DOI: https://dx.doi.org/10.3748/wjg.v13.i2.257