Combination of small interfering RNA and lamivudine on inhibition of human B virus replication in HepG2.2.15 cells

Table 1 Concentration of HBeAg and HBsAg in HepG2.2.15 cells detected by ELISA

	HBeAg			HBsAg
Group	48 h	72 h	96 h	48 h	72 h	96 h
siRNA	6.54 ± 0.30 (15.43)	6.23 ± 0.27 (55.78)	4.90 ± 0.29 (70.92)a	6.94 ± 0.14 (13.88)	14.00 ± 0.64 (15.06)	7.68 ± 0.70 (60.57)a
LAM	6.80 ± 0.22 (10.48)	10.02 ± 0.45 (15.20)	9.53 ± 0.44 (22.85)a	7.10 ± 0.19 (11.03)	14.39 ± 0.45 (12.38)	11.93 ± 0.36(30.53)a
siRNA+LAM	6.01 ± 0.42 (25.52)	8.40 ± 0.20(32.55)	2.89 ± 0.48 (91.8)ac	6.47 ± 0.27 (22.24)	11.26 ± 0.77 (34.62)	4.59 ± 0.57(82.40)ac
HK	7.25 ± 0.27 (0)	11.06 ± 0.21 (0)	10.92 ± 0.33 (0)	7.52 ± 0.18 (0)	15.93 ± 0.44(0)	15.43 ± 0.47 (0)
psi/U6	7.35 ± 0.34	11.44 ± 0.17	11.73 ± 0.38	7.72 ± 0.22	16.11 ± 0.41	16.25 ± 0.48

The inhibitory ratio is shown in bracket.

^aP < 0.05 vs HK or psi/U6 group;

^cP < 0.05 vs siRNA or LAM group.

Table 2 Real-time PCR detection of the level of HBV DNA in HepG2.2.15 cells

Group	48 h	72 h	96 h
siRNA	7.02 ± 0.33 (7.63)	5.69 ± 0.18 (31.86)	3.10 ± 0.27 (65.48)a
LAM	6.03 ± 0.41 (20.66)	5.19 ± 0.18 (37.84)	2.40 ± 0.33 (73.27)a
siRNA + LAM	5.60 ± 0.37 (26.32)	4.72 ± 0.21 (43.47)	1.04 ± 026 (88.42)ac
HK	7.50 ± 0.33 (0)	8.26 ± 0.20 (0)	8.65 ± 0.21 (0)
psil/U6	7.60 ± 031	8.35 ± 0.33	8.98 ± 0.33

The inhibitory ratio is shown in bracket.

^aP < 0.05 vs HK or psi/U6 group;

^cP < 0.05 vs siRNA or LAM group.