Mechanism of apoptotic effects induced selectively by ursodeoxycholic acid on human hepatoma cell lines

Table 1 UDCA induces apoptosis of HepG2, BEL7402 and L-02 cell lines (means ± SD, n = 3)

UDCA mmol/L	Apoptosis rate (%)
	HepG2	BEL7402	L-02
0 (control)	2.58 ± 0.26	2.02 ± 0.13	3.03 ± 0.12
0.1	8.4 ± 1.6b	9.3 ± 2.0b	3.11 ± 0.21a
0.4	19.0 ± 3.0b	17.2 ± 1.7b	3.66 ± 0.13a
0.8	36.0 ± 6.0b	37.0 ± 5.0b	4.29 ± 0.23a
1.0	42.0 ± 6.0b	44.0 ± 4.0b	4.58 ± 0.18a

^aP > 0.05,

^bP < 0.01, vs control.

Table 2 Effect of UDCA on expression of bcl-2 and Bax in HepG2, BEL7402 and L-02 (means ± SD, n = 4)

	HepG2 (%)		BEL7402 (%)		L-02 (%)
UDCA (mmol/L)	bcl-2	Bax	bcl-2	Bax	bcl-2	Bax
0 (control)	24.3 ± 2.4	43.0 ± 5.0	21.6 ± 1.8	44.0 ± 4.0	1.0 ± 0.2	62.0 ± 4.0
0.2	19.4 ± 1.5c	42.0 ± 4.0a	20.1 ± 1.9a	48.4 ± 2.5a	1.0 ± 0.1a	61.0 ± 5.0a
0.4	14.2 ± 2.1b	53.6 ± 2.5c	13.5 ± 1.8b	56.0 ± 4.0c	0.8 ± 0.1a	59.0 ± 3.0a
0.8	10.1 ± 1.6b	59.0 ± 3.0b	11.6 ± 2.1b	59.0 ± 3.0b	0.6 ± 0.3a	64.0 ± 4.0a

^aP > 0.05,

^bP < 0.01,

^cP < 0.05, vs control.