Cloning of α-β fusion gene from Clostridium perfringens and its expression

Table 1 Detection of the fusion protein by ELISA assay

Antibody	pZCPAB	pBV221	pXETA	pXETB
Anti-α-toxin	0.66 ± 0.08	0.001	0.92 ± 0.13
Anti-β-toxin	0.72 ± 0.12	0.002		0.97 ± 0.19

Table 2 Expressed α-β fusion protein in mice

Challengedose	α-toxin(survived mice/total mice)Control group Treatment group		β-toxin(survived mice/total mice)Control group Treatment group
1 MLD	0/10	10/10	0/10	10/10
2 MLD	0/10	8/10	0/10	9/10