Expression patterns of cytokine, growth factor and cell cycle-related genes after partial hepatectomy in rats with thioacetamide-induced cirrhosis

Table 1 Primers for reverse-transcription-PCR of HGF, TGF-α, p21, p27 and p53 mRNAs and 28S rRNA.

Target sequence1	Primer	Sequence (5’- 3’)
28S	28S Forward	GGC CAA GCG TTC ATA GCG AC
	28S Reverse	GAG GCG TTC AGT CAT AAT CC
HGF[38]	HGF Forward	CCC GGT GCT GCA GCA TGT CCT
	HGF Reverse	TCC CCT CGA TTT CGA CAG
TGF-α[38]	TGF-α Forward	GAC AAG TTG AAC AAG AAC CTC
	TGF-α Reverse	CGT CAT CCA CCT AAT ACA TAA G
p21[52]	p21 Forward	ATG TCC GAT CCT GGT GAT GTC
	p21 Reverse	CAC TTC AGG GCT TTC TCT TGC
p27[52]	p27 Forward	GCA GCT TGC CCG AGT TCT AC
	p27 Reverse	TTC TTG GGC GTC TGC TCC AC
p53[53]	p53 Forward	TCC TCC CCA ACA TCT TAT CC
	p53 Reverse	GCA CAA ACA CGA ACC TCA AA

¹The 28 S primers were kindly provided by Assoc. Prof. Chang Chan Fong (Department of Biochemistry, National University of Singapore). The other primers were as previously reported[38,52,53].