Rapid Communication
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Nov 14, 2006; 12(42): 6874-6878
Published online Nov 14, 2006. doi: 10.3748/wjg.v12.i42.6874
Table 1 List of primary antisera used in the present study
AntiserumCodeDilutionSource
CGRPsc-289201:200Santa Cruz Biotech Inc.
Substance Psc-97581:200Santa Cruz Biotech Inc.
VIPNCL-VIPp1:200Nova Castra Lab, UK
BombesinNCL-BOMp1:200Nova Castra Lab, UK
Somatostatin14CL-SOMATOp1:200Nova Castra Lab, UK
Secretinsc-209381:200Santa Cruz Biotech Inc.
Neurotensinsc-208061:200Santa Cruz Biotech Inc.
NPYsc-289431:200Santa Cruz Biotech Inc.
TrkAsc-1181:200Santa Cruz Biotech Inc.
TrkBsc-121:200Santa Cruz Biotech Inc.
TrkCsc-1171:200Santa Cruz Biotech Inc.
Table 2 Mean number of endocrine cells per intestinal fold (mean ± SD) in flower fish (Pseudophoxinus antalyae) (200 intestinal folds were examined for each antisera)
RegionCalcitonin gene related peptideSubstance-PVasoactive intestinal polypeptideBombesinSomatostatin-14SecretinTrkATrkBTrkCNeuropeptide YNeurotensinAnova(F)P
The enlarged area after oesophagus5.00 ± 1.5504.75 ± 2.21720.50 ± 9.8833.75 ± 2.5008.50 ± 9.0373.50 ± 1.2914.00 ± 4.3593.00 ± 1005.67 ± 3.2150.67 ± 0.5774.33 ± 3.2154.4560.001
Anterior intestine5.25 ± 2.5002.75 ± 0.95712.00 ± 6.4812.25 ± 1.2582.75 ± 1.5004.25 ± 2.0620.00 ± 0.0002.67 ± 2.8871.00 ± 1.0000.00 ± 0.0001.33 ± 0.5776.1860.000
Middle intestine5.25 ± 2.2174.25 ± 0.95712.25 ± 6.7021.00 ± 0.8167.75 ± 1.7084.75 ± 1.7080.00 ± 0.0001.67 ± 1.5281.00 ± 1.0000.67 ± 0.5771.00 ± 1.0008.1150.000
Posterior intestine4.50 ± 2.3806.75 ± 2.2177.25 ± 2.2173.00 ± 0.8160.00 ± 0.0002.00 ± 1.440.00 ± 0.0000.67 ± 1.1550.67 ± 1.1550.00 ± 0.0000.33 ± 0.57712.8290.000