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©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Nov 14, 2006; 12(42): 6806-6811
Published online Nov 14, 2006. doi: 10.3748/wjg.v12.i42.6806
Published online Nov 14, 2006. doi: 10.3748/wjg.v12.i42.6806
Table 1 Taqman primers and probes for real-time quantitative PCR
| Gene | Primer/Probe sequences | Position | Size |
| (Accession No.) | (Forward/Reverse/Probe) | (nt No.) | (bp) |
| PAP I | F 5'-AAAATACCCTCTGCACGCATTAG-3' | 153-171 | 67 |
| (NM_053289) | R 5'-GGGCATAGCAGTAGGAGCCATA-3' | 219-198 | |
| P 5'-FAM-TGCCCCAAAGGCTCCCAGGC-TAMRA-3' | 177-196 | ||
| PAP II | F 5'-CCAGAAGGCAGTGCCCTCTA-3' | 240-259 | 67 |
| (L10229) | R 5'-GCAGTAAGAACGATAAGCCTTGGA-3' | 306-283 | |
| P 5'-FAM-ACGAACCAGCTGCCCCATGG-TAMRA-3' | 261-280 | ||
| PAP III | F 5'-TGTGCCCACTTCACGTATCAG-3' | 121-140 | 64 |
| (L_20869) | R 5'-GGCATAGCAATAGGAGCCATAGG-3' | 184-162 | |
| P 5'-FAM-TGCCCCAAGGGCTCGCG-TAMRA-3 | 143-159 | ||
| β-actin | F 5’-TTCAACACCCCAGCCATGT-3’ | 379-397 | 68 |
| R 5’-GTGGTACGACCAGAGGCATAC A-3’ | 446-425 | ||
| P 5’- FAM-CGTAGCCATCCAGGCTGTGTTGTCC-MGBNFQ-3’ | 399-422 |
- Citation: Kandil E, Lin YY, Bluth MH, Zhang H, Levi G, Zenilman ME. Dexamethasone mediates protection against acute pancreatitis via upregulation of pancreatitis-associated proteins. World J Gastroenterol 2006; 12(42): 6806-6811
- URL: https://www.wjgnet.com/1007-9327/full/v12/i42/6806.htm
- DOI: https://dx.doi.org/10.3748/wjg.v12.i42.6806