Expression of β2-integrin on leukocytes in liver cirrhosis

Table 1 Clinical characteristics of patients with liver cirrhosis

Characteristic	Child-pugh B	Child-pugh C
Encephalopathy (n)	2	10
Ascites (n)	9	19
Median bilirubin (range) (mg/L)	34 (9–78)	69 (22–139)
Median albumin (range) (g/L)	29 (22–33)	22 (19–29)
Median prothrombin time (range) (s)	19 (17.2–23.3)	23 (18.2–26.8)

Table 2 β2-integrin expression on peripheral blood leukocytes and sICAM-1 and TNF-α serum concentrations in liver cirrhosis (stages B and C) and in healthy subjects

Leukocytes	Receptores	Healthy subjects(n = 20)	Liver	cirrhosis
			Stage B(n = 21)	Stage C(n = 19)
	CD14 (%)	26.2 ± 2.3	26.1 ± 6.3	26.8 ± 3.9
Monocytes	MIF1	2.9 ± 1.5	8.7 ± 6.9	8.1 ± 6.1
	CD11a (%)	99.9 ± 0.09	99.9 ± 0.08	99.9 ± 0.8
	MIF1	14.8 ± 2.4	15.1 ± 1.8a	16.3 ± 1.7b
	CD11b (%)	99.8 ± 0.08	99.5 ± 0.7	99.8 ± 0.2
	MIF1	18.4 ± 4.6	18.7 ± 4.8a	22.2 ± 4.4a
	CD11c (%)	99.5 ± 0.5	97.5 ± 4.5	96.6 ± 7.0
	MIF1	6.5 ± 1.3	7.0 ± 1.5	7.4 ± 2.1
	CD49d (%)	63.8 ± 14.7	66.9 ± 22.5	66.2 ± 21.2
	MIF1	1.39 ± 0.43	1.85 ± 0.72	1.52 ± 0.68
Granulocytes	CD11a (%)	99.3 ± 0.5	98.0 ± 4.8	94.7 ± 0.5
	MIF1	4.4 ± 0.4	4.3 ± 0.5	4.4 ± 0.7
	CD11b (%)	99.5 ± 1.1	99.5 ± 0.7	98.6 ± 2.7
	MIF1	10.5 ± 2.4	10.0 ± 2.8a	12.1 ± 2.6
	CD11c (%)	87.0 ± 5.0	80.8 ± 11.4	71.9 ± 12.4
	MIF1	2.8 ± 0.4	2.7 ± 0.7	3.0 ± 0.9
	CD49d (%)	4.4 ± 2.4	4.6 ± 3.0	6.3 ± 3.9
	MIF1	0.13 ± 0.01	0.17 ± 0.05b	0.17 ± 0.03b
Lymphocytes	CD11a (%)	98.1 ± 0.8	97.2 ± 1.7	97.6 ± 1.3
	MIF1	8.0 ± 1.2	9.7 ± 1.6b	9.9 ± 2.0b
	CD11b (%)	23.7 ± 5.3	24.5 ± 5.2	24.0 ± 8.0
	MIF1	0.56 ± 0.12	0.61 ± 0.18a	0.75 ± 0.24
	CD11c (%)	25.2 ± 6.6	23.2 ± 5.6	29.4 ± 7.3
	MIF1	0.77 ± 0.12	0.91 ± 0.33	1.0 ± 0.25b
	CD49d (%)	31.2 ± 12.5	37.9 ± 12.1	38.8 ± 11.8
	MIF1	0.48 ± 0.12	0.68 ± 0.34b	0.63 ± 0.18b
sICAM-1 (μg/L)		254 ± 74	839 ± 34b	957 ± 328b
TNFα (μg/L)		1.58 ± 0.22	2.89 ± 1.34b	2.98 ± 1.42b

¹Medial intensity of fluorescence;

^aP < 0.05 vs Child-Pugh C stage,

^bP < 0.01 vs healthy subjects (Mann-Whitney U-test).

Table 3 The correlation of β2-integrin expression on leukocytes and sICAM level in blood sera from liver cirrhosis (stages B and C)

	CD11a	CD11b	CD11c	CD49d
			Monocytes
Monocytes CD11a		H1: r = 0.6, P < 0.01	B2: r = 0.39, P < 0.05 C3: r = 0.56, P < 0.01 H1: r = 0.73, P < 0.01	NS4
			Granulocytes
	NS4	NS4	NS4	NS4
			Lymphocytes
	B2: r = 0.56, P < 0.01	NS4	NS4	NS4
Granulocytes			Monocytes
CD11a	NS4	NS4	C3: r = 0.47, P < 0.04	NS4
			Granulocyte
			C3: r = 0.42, P < 0.01	C3: r = 0.53, P < 0.01
			H1: r = 0.54, P < 0.05
			Lymphocytes
	NS4	NS4	NS4	NS4
Lymphocytes			Monocytes
CD11a	NS4	NS4	NS4	NS4
			Granulocytes
	NS4	NS4	NS4	NS4
			Lymphocytes
		C3: r = 0.51, P < 0.02	NS4	C3: r = 0.51, P < 0.02

¹Healthy subjects;

²B stage of liver cirrhosis;

³C stage of liver cirrhosis;

⁴Not significant. Spearman test.