Increased DNA binding activity of NF-kB, STAT-3, SMAD3 and AP-1 in acutely damaged liver

Table 1 Oligonucleotide sequences used for PCR amplification

Gene	Sequence	Annealing temperature	Cycles	Size (bp)
HPRT	5’ TCC CAG CGT CGT GAT TAG TG 3’	60°C	30	618
	5’ GGC TTT TCC ACT TTC GCT GA 3’
iNOS	5’ TAG AGG AAC ATC TGG CCA GG 3’	58°C	25	255
	5’ TGG CCG ACC TGA TGT TGC CA 3’
c-MET	5’ CAG TGA TGA TCT CAA TGG GCA AT 3’	60°C	28	725
	5’ AAT GCC CTC TTC CTA TGA CTT C 3’
HGF	5’ AGC TCA GAA CCG ACC GGC TTG CAA CAG GAT3’	60°C	27	618
	5’ TTA CCA ATG ATG CAA TTT CTA ATA TAG TCT3’

Table 2 Oligonucleotide sequences used for EMSA

Transcription factor	Sequence
NF-κB	5´ AGT TGA GGG GAC TTT CCC AGG C 3´
	3´ TCA ACT CCC CTG AAA GGG TCC G 5´
STAT-3	5´ GAT CCT TCT GGG AAT TCC 3´
	3´ CTA GGA AGA TCC TTA AGG 5´
SMAD-3	5´ TCG AGA GC CAGA CAA AAA GC CAGA CAT TTA GC CAGA CAC 3´
	3´ AGC TCT CG GTCT GTT TTT CG GTCT GTA AAT CG GTCT GTG 5´
AP-1	5´ GAT CGA TGA CTC AGA GGA AAA 3´
	3´ CTA GCT ACT GAG TCT CCT TTT 5´

Bold and underlined letters denote specific consensus DNA-binding sequences.