Expressions of sonic hedgehog, patched, smoothened and Gli-1 in human intestinal stromal tumors and their correlation with prognosis

Table 1 Aberrant expression of Hedgehog pathway signaling proteins in intestinal stromal tumors n (%)

			Shh		Ptc		Smo		Gli-1
		n	+	-	+	-	+	-	+	-
GIST		31	18 (58.1)	13 (41.9)	24 (77.4)	7 (22.6)	25 (80.6)	6 (19.4)	18 (58.1)	13 (41.9)
I	GIST, M	7 (22.6)	4 (57.1)	3 (42.9)	5 (71.4)	2 (28.6)	6 (85.7)	1 (14.3)	4 (57.1)	3 (42.9)
	GIST, N	8 (25.8)	5 (62.5)	3 (37.5)	5 (62.5)	3 (37.5)	4 (50.0)	4 (50.0)	3 (37.5)	5 (62.5)
	GIST, UN	16 (51.6)	9 (56.3)	7 (43.8)	14 (87.5)	2 (12.5)	15 (93.8)	1 (6.3) c	11 (68.8)	5 (31.3)
II	GIST, EP	5 (16.1)	3 (60.0)	2 (40.0)	4 (80.0)	1 (20.0)	3 (60.0)	2 (40.0)	2 (40.0)	3 (60.0)
	GIST, MIX	3 (9.7)	2 (66.7)	1 (33.3)	2 (66.7)	1 (33.3)	3 (100)	0 (0.0)	2 (66.7)	1 (33.3)
	GIST, SP	23 (74.2)	13 (56.5)	10 (43.4)	17 (73.9)	6 (26.1)	19 (82.6)	4 (17.4)	14 (60.9)	9 (39.1)
Leiomyoma		13	12 (92.3)	1 (7.7) a	12 (92.3)	1 (7.7)	9 (69.2)	4 (30.8)	12 (92.3)	1 (7.7) a
Schwannoma		6	5 (83.3)	1 (16.7)	5 (83.3)	1 (16.7)	5 (83.3)	1 (16.7)	6 (100)	0 (0.0)

n (%): Tumor cases followed by percentage (%) of total cases.

^aP < 0.05 between Leiomyoma and GIST in Shh or Gli-1;

^cP < 0.05 between GIST, UN and GIST, N.

Table 2 Expression of Hedgehog pathway components in various categories of intestinal stromal tumors n (%)

		Shh		Ptc		Smo		Gli-1
	n	+	-	+	-	+	-	+	-
GIST	31	18 (58.1)	13 (41.9)	24 (77.4)	7 (22.6)	25 (80.6)	6 (19.4)	18 (58.1)	13 (41.9)
Risk categories		P < 0.05		NS		NS		NS
High	4	1 (25.0)	3 (75.0)	3 (75.0)	1 (25.0)	3 (75.0)	1 (25.0)	3 (75.0)	1 (25.0)
Intermediate	6	3 (50.0)	3 (50.0)	5 (83.3)	1 (16.7)	4 (66.7)	2 (33.3)	2 (33.3)	4 (66.7)
Low	16	9 (56.3)	7 (43.8)	11 (68.8)	5 (31.3)	13 (81.3)	3 (18.8)	9 (56.3)	7 (43.8)
Very low	5	5 (100)	0 (0.0)	5 (100)	0 (0.0)	5 (100)	0 (0.0)	4 (80.0)	1 (20.0)
Tumor size (cm in diameter)		P < 0.05		NS		NS		NS
n ≤ 2	8	7 (87.5)	1 (12.5)	7 (87.5)	1 (12.5)	8 (100)	0 (0.0)	6 (75.0)	2 (25.0)
2 < n ≤ 5	18	10 (55.6)	8 (44.4)	14 (77.8)	4 (22.2)	13 (72.2)	5 (27.8)	8 (44.4)	10 (55.6)
5 < n	5	1 (20.0)	4 (80.0)	3 (60.0)	2 (40.0)	4 (80.0)	1 (20.0)	4 (80.0)	1 (20.0)
Mitosis counts1		NS		NS		NS		NS
0-5	23	13 (56.5)	10 (43.5)	17 (73.9)	6 (26.1)	18 (78.3)	5 (21.7)	14 (60.9)	9 (39.1)
6-10	3	2 (66.7)	1 (33.3)	3 (100)	0 (0.0)	3 (100)	0 (0.0)	2 (66.7)	1 (33.3)
11-28	5	3 (60.0)	2 (40.0)	4 (80.0)	1 (20.0)	4 (80.0)	1 (20.0)	2 (40.0)	3 (60.0)

n (%): Tumor cases followed by percentage (%) of total cases; NS: Not significant;

¹Numbers per 50 areas in high-power field (× 400).