Prevalence of SLC22A4, SLC22A5 and CARD15 gene mutations in Hungarian pediatric patients with Crohn’s disease

Table 1 Primer sequences and annealing temperatures for genotypings

	SNP	Primers	Tannealing (°C)
NOD2/	R702W	F: GAGCCGCACAACCTTCAGATC	50
CARD15		R: ACTTGAGGTGCCCAACATTCAG
	G908R	F: GTTCATGTCTAGAACACATATCAGG	50
		R: GTTCAAAGACCTTCAGAACTGG
	1007finsC	F: CCTTGAAGCTCACCATTGTATC	50
		R: GATCCTCAAAATTCTGCCATTC
OCTN1	C1672T	F: AGAGAGTCCTCCTATCTGATTG	54
		R: TCCTAGCTATTCTTCCATGC
OCTN2	G-207C	F: AGTCCCGCTGCCTTCCTAAG	58
		R: GTCACCTCGTCGTAGTCCCG

Table 2 Comparison of the alleles of OCTN cation transporters and NOD2/CARD15 genes in pediatric and adult Crohn’s disease patients with controls

		Pediatric patients	Adult patients	Controls children
		n =19 (%)	n = 55 (%)	n = 49 (%)
CARD15 genotype
R702W	CC	18 (94.7)	46 (83.6)	46 (93.9)
	CT	1 (5.3)	8 (14.6)	2 (4.1)
	TT	-	1 (1.8)	1 (2.0)
	T allele frequency (%)	2.63	9.09	4.08
G908R	GG	14 (73.7)	53 (96.4)	48 (98.0)
	GC	3 (15.8)	2 (3.6)	1 (2.0)
	CC	2 (10.5)	-	-
	C allele frequency (%)	18.4	1.82	1.02
1007finsC	- -	13 (68.5)	44 (80.0)	46 (93.9)
	- insC	4 (21.0)	9 (16.4)	3 (6.1)
	insC insC	2 (10.5)	2 (3.6)	-
	Cins allele frequency (%)	21.1%	11.8%	3.06%
SLC22A4 genotype
C1672T	CC	4 (21.0)	18 (32.7)	12 (24.5)
	CT	11 (58.0)	30 (54.5)	25 (51.0)
	TT	4 (21.0)	7 (12.8)	12 (24.5)
	T allele frequency (%)	50.0	40.0	50.0
SLC22A5 genotype
G-207C	GG	3 (15.8)	14 (25.5)	10 (20.4)
	GC	7 (36.8)	31 (56.4)	26 (53.1)
	CC	9 (47.4)	10 (18.1)	13 (26.5)
	C allele frequency (%)	65.8	46.4	53.1