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©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 7, 2006; 12(33): 5375-5378
Published online Sep 7, 2006. doi: 10.3748/wjg.v12.i33.5375
Published online Sep 7, 2006. doi: 10.3748/wjg.v12.i33.5375
Table 1 Detection of H pylori status by Western blot, ELISA and CagA ELISA of IgA
Patients | H pylori lgA | Toatl | ||||||
Western blot n (%) | ELISA n (%) | CagA ELISA n (%) | ||||||
+ | - | ± | + | - | + | - | ||
H pylori (+) (n = 48) | 17 (89.5) | 24 (80.0) | 7 (100) | 26 (96.3) | 22 (75.9) | 16 (94.1) | 32 (82.1) | 48 (85.7) |
H pylori (-) (n = 8) | 2 (10.5) | 6 (20.6) | 0 | 1 (3.7) | 7 (24.1) | 1 (5.9) | 7 (17.9) | 8 (14.3) |
Total | 19 | 30 | 7 | 27 | 29 | 17 | 39 | 56 |
Table 2 Detection of H pylori status by Western blot, ELISA and CagA ELISA of IgG
Patients | H pylori lgG | Toatl | ||||||
Western blot n (%) | ELISA n (%) | CagA ELISA n (%) | ||||||
+ | - | ± | + | - | + | - | ||
H pylori (+) (n = 48) | 40 (88.9) | 1 (50.0) | 7 (77.8) | 42 (91.3) | 6 (85.7) | 26 (92.9) | 22 (78.6) | 48 (85.7) |
H pylori (-) (n = 8) | 5 (11.1) | 1 (50.0) | 2 (22.2) | 4 (8.7) | 4 (57.1) | 2 (7.1) | 6 (21.4) | 8 (14.3) |
Total | 45 | 2 | 9 | 46 | 10 | 28 | 28 | 56 |
Table 3 Results of anti-H pylori ELISA and CagA ELISA of IgA, IgG in dyspeptic patients
Sensitivity% | Specificity% | PPV% | NPV% | LR + | LR - | Diagnosticaccuracy % | |
ELISA IgA | 54.2 | 87.5 | 96.3 | 24.1 | 0.62 | 4.33 | 58.9 |
ELISA IgG | 87.5 | 50 | 91.3 | 40 | 1.75 | 1.75 | 82.1 |
CagA ELISA IgA | 33.3 | 87.5 | 94.1 | 18 | 0.38 | 2.67 | 41.1 |
CagA ELISA IgG | 54.2 | 75 | 92.9 | 21.4 | 0.72 | 2.17 | 57.1 |
Table 4 Anti-H pylori IgG Western blot results of the distribution of different antigens
Antigens | Positive patients (n = 56) | % |
p120 (CagA) | 28 | 50 |
p95 (VacA) | 35 | 62.5 |
p75 | 18 | 32.1 |
p67 (FSH) | 23 | 41.1 |
p66 (UreB) | 49 | 87.5 |
p57 (HSP homolog) | 37 | 66.1 |
p54 (Flagellin) | 24 | 42.9 |
p50 | 24 | 42.9 |
p41 | 28 | 50 |
p33 | 13 | 23.2 |
p30 | 24 | 42.9 |
p29 (UreA) | 45 | 80.4 |
p26 | 23 | 41.1 |
p19 (OMP) | 32 | 57.1 |
p17 | 20 | 35.7 |
- Citation: Yilmaz Ö, Şen N, Küpelioğlu AA, Şimşek İ. Detection of H pylori infection by ELISA and Western blot techniques and evaluation of anti CagA seropositivity in adult Turkish dyspeptic patients. World J Gastroenterol 2006; 12(33): 5375-5378
- URL: https://www.wjgnet.com/1007-9327/full/v12/i33/5375.htm
- DOI: https://dx.doi.org/10.3748/wjg.v12.i33.5375