Effect of Fructus Psoraleae on motility of gallbladder isolated smooth muscle strips from guinea pigs

Table 1 Effect of Fructus Psoraleae on resting tension (g) of isolated gallbladder muscle strip after pretreated with antagonists (means ± SD)

Resting tension (g)	Fructus Psoraleae (g/L)
	0	0.1	0.3	1	3	10	20
Pso	0.00 ± 0.00	0.00 ± 0.00	0.02 ± 0.04	0.09 ± 0.04	0.24 ± 0.08a	0.88 ± 0.35d	1.37 ± 0.41d
Ben + Pso	0.00 ± 0.00	0.00 ± 0.00	0.02 ± 0.04	0.07 ± 0.06	0.14 ± 0.08c	0.29 ± 0.17bh	0.45 ± 0.38dh
Phe + Pso	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.04	0.07 ± 0.07	0.17 ± 0.08b	0.46 ± 0.11dh	0.89 ± 0.22df
4-DAMP + Pso	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.04f	0.09 ± 0.07f	0.30 ± 0.18dh	0.70 ± 0.35dh
Hex + Pso	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.05 ± 0.08	0.16 ± 0.14a	0.42 ± 0.20ch	0.94 ± 0.23cd
Ido + Pso	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.02	0.09 ± 0.06	0.21 ± 0.12a	0.71 ± 0.23d	1.31 ± 0.41d
Iso + Pso	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.03f	0.10 ± 0.06f	0.38 ± 0.19ch	0.94 ± 0.26cd

^aP < 0.05,

^bP < 0.01,

^dP < 0.001 vs control (under 1-g initial load the gallbladder spontaneous contraction when Fructus Psoraleae was 0 g/L ). The resting tension of each strip in control was 0 (n = 8).

^cP < 0.05,

^fP < 0.01,

^hP < 0.001 vs Fructus Psoraleae (the resting tension of adding each concentration of Fructus Psoraleae) (n = 8). Pso: Fructus Psoraleae; Ben: Benzhydramine; Phe: Phentolamine; Hex: Hexamethonium; Ido: Idomethine; Iso: Verapamil.

Table 2 Effect of Fructus Psoraleae on the contractile amplitude (mm) of isolated gallbladder muscle strip after pretreated with antagonists (means ± SD)

Amplitude (mm)	Fructus psoraleae (g/L)
	0	0.1	0.3	1	3	10	20
Pso	4.54 ± 0.64	4.23 ± 0.78	4.24 ± 0.84	4.07 ± 0.72	3.71 ± 0.59a	2.36 ± 0.77d	1.72 ± 0.74d
Ben + Pso	4.70 ± 0.57	4.43 ± 0.80	4.19 ± 0.88	4.09 ± 0.97	3.93 ± 0.97	3.55 ± 0.93a	2.37 ± 0.83d
Phe + Pso	4.71 ± 1.37	4.47 ± 1.42	4.64 ± 1.32	4.49 ± 1.35	4.17 ± 1.41	3.36 ± 1.43a	2.15 ± 0.65d
4-DAMP + Pso	4.91 ± 0.60	4.58 ± 0.71	4.23 ± 0.60	4.27 ± 0.84	3.84 ± 0.81a	3.70 ± 1.28b	2.34 ± 1.07d
Hex + Pso	5.48 ± 1.57	5.13 ± 1.64	5.34 ± 1.60	4.86 ± 1.63	4.80 ± 1.46	3.43 ± 1.10b	1.93 ± 1.02d
Ido + Pso	5.40 ± 1.30	5.01 ± 1.37	4.83 ± 1.33	4.62 ± 1.42	4.16 ± 1.29	3.39 ± 1.48b	2.24 ± 1.25d
Iso + Pso	4.47 ± 1.23	4.42 ± 1.37	4.60 ± 1.56	4.29 ± 1.32	4.00 ± 1.08	3.98 ± 1.74	2.80 ± 1.57b

^aP < 0.05,

^bP < 0.01,

^dP < 0.001 vs control (under 1-g initial load the gallbladder spontaneous mean contraction amplitude when Fructus Psoraleae was 0 g/L ) ( n = 8).

Table 3 Effect of Fructus Psoraleae on the contractile frequency(waves/min) of isolated gallbladder muscle strip after pretreated with antagonists (means ± SD)

Frequency (w/min)	Fructus psoraleae (g/L)
	0	0.1	0.3	1	3	10	20
Pso	3.3 ± 0.5	3.3 ± 0.7	3.3 ± 0.6	3.8 ± 0.9	4.6 ± 0.7b	7.3 ± 1.2d	8.3 ± 1.2d
Ben + Pso	3.4 ± 0.5	3.6 ± 0.4	3.6 ± 0.4	3.7 ± 0.4	4.6 ± 0.4d	6.3 ± 0.5d	7.3 ± 0.8d
Phe + Pso	3.5 ± 0.7	3.9 ± 0.8	3.9 ± 0.8	4.4 ± 1.0	5.1 ± 0.8b	7.1 ± 1.2d	9.1 ± 1.1d
4-DAMP + Pso	3.5 ± 0.4	3.5 ± 0.4	3.6 ± 0.6	3.9 ± 0.7	4.2 ± 0.5a	5.1 ± 0.9dh	6.8 ± 0.5df
Hex + Pso	3.3 ± 0.5	3.5 ± 0.8	3.5 ± 0.5	3.9 ± 0.8	4.8 ± 1.0d	6.0 ± 0.7cd	7.0 ± 0.9cd
Ido + Pso	3.4 ± 0.5	3.4 ± 0.6	3.6 ± 0.7	3.8 ± 0.7	4.4 ± 0.7a	6.9 ± 1.4d	8.4 ± 1.1d
Iso + Pso	3.4 ± 0.5	3.4 ± 0.8	3.5 ± 0.8	3.8 ± 0.9	5.2 ± 1.4b	6.3 ± 1.2d	8.8 ± 1.6d

^aP < 0.05,

^bP < 0.01,

^dP < 0.001 vs control (under 1-g initial load the gallbladder spontaneous contraction frequency when Fructus Psoraleae was 0 g/L ) (n = 8).

^cP < 0.05,

^fP < 0.01,

^hP < 0.001 vs Fructus Psoraleae (contraction frequency of adding each concentration of Fructus Psoraleae) (n = 8).