Analysis of differentially expressed proteins in cancerous and normal colonic tissues

Table 1 Clinical and pathological data of patient tissue specimens

Patient #	Age	Gender	Race	Cancer stage	Cancer grade
MMN	77	Female	Malay	Stage IV	Well differentiated adenocarcinoma
LKH	66	Male	Chinese	Stage II	Moderately differentiated adenocarcinoma
CCH	55	Male	Chinese	Stage IV	Moderately differentiated adenocarcinoma
NSC	55	Female	Chinese	Stage III	Moderately differentiated adenocarcinoma

Table 2 Up-regulated, down-regulated proteins extracted in S1, S2, S3 buffers from colon tissues

SWISS-PROTaccession number	Down-regulated proteins(Normal tissues)	SWISS-PROTaccession number	Up-regulated proteins (Cancer tissues)
P12718	Actin, gamma-enteric smooth muscle	P04262	Type II, keratin subunit protein
P17661	Desmin	P35900	Keratin 20
P08670	Vimetin	P35908	Keratin, 67k type II epidermal
Q9DFD0	Hypothetical protein	P13647	Keratin k5
Q7Z5W1	Hypothetical protein	Q86Y46	Keratin 6 irs 3
CAD19027	Hypothetical protein	P04264*	Keratin 1, type II cytoskeletal
BAC87457	Hypothetical protein	P35527	Cytoskeletal 9
CAC08866	Hypothetical protein	P04264	Keratin, type II cytoskeletal I
CAC08835	Hypothetical protein	P35527	Keratin 9, type I, cytoskeletal
Q8WTU6	Hypothetical protein	P08779	Keratin, type 1, cytoskeletal 16
Q01995	Transgelin	P48669	Keratin, type II, cytoskeletal 6F
Q91706	Skeletal muscle beta-tropomyosin	P02538	Keratin 6A, type II
P12277	Creatine kinase	P19013	Keratin, type II, cytoskeletal 4
P00915	Actin-gamma	P08729	Keratin, type II, cytoskeletal 7
Q96QE3	ATP[GTP]-binding protein	P12035	Keratin, type II, cytoskeletal 3
		P05783	Keratin, type I, cytoskeletal 18
		P13645	Keratin, type I, cytoskeleton 10
		Q8NF17	Hypothetical protein
		CAE99935	Hypothetical protein
		P02675	Fibrinogen beta chain
		P00915	Carbonate dehydratase EC 4.2.1.1
		Q9PTK9	Kinesin-like protein 2
		P60174	Triosephosphate isomerase EC. 5.3.1.1
		Q6PJ43	ACTGI protein
		Q13733	Sodium/potassium-transporting ATPase alpha-4 chain EC 3.6.3.9

Table 3 Unique proteins extracted in S1, S2, S3 buffers from colon tissues

SWISS-PROTaccession number	Unique proteins (Normal tissues)	SWISS-PROTaccession number	Unique proteins (Cancer tissues)
P32119	Peroxiredoxin 2 precursor	Q9BX84	LTRPC6 channel kinase 6
Q03001	Bullous pemphigoid antigen 1 precursor	Q9AVW8	DNA-directed RNA polymerase II largest chain EC 2.7.7.6
P01009	Alpha-1-antitrypsin precursor	P29312	14-3-3 protein zeta
P20848	Alpha-1-antitrypsin related protein presursor	P31946	14-3-3 protein beta/alpha
1QMBA	Alpha-1-antitrypsin mutant YES, chain A	S38532	Protein 14-3-3 eta chain
		S31975	14-3-3 protein epsilon