Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells

Table 1 Evaluation of effects of four culture systems on ex vivo expansion of hematopoietic progenitors in short-term serum-free culture

Culture systems	Total nucleated cells	CD34+ cells	CFU-C	CFU-GEMM
tfhMSCs coculture system	87.56 ± 9.51b	12.39 ± 2.34b	40.59 ± 6.23a	15.84 ± 3.96b
hMSCs coculture system	53.23 ± 6.79a	9.87 ± 2.89a	38.78 ± 4.28a	10.57 ± 2.58b
Cytokines culture system	46.25 ± 4.89	9.58 ± 1.26a	27.68 ± 3.32	7.19 ± 1.96
hMSCs (TPO/FL-free) culture system	48.52 ± 6.49	6.79 ± 0.62	32.85 ± 4.35	5.67 ± 1.09

^aP < 0.05,

^bP < 0.01 vs as compared with hMSCs (TPO/FL-free) culture system.

Table 2 Results of LTC-IC assay using UCB CD34+ cells or those generated by four culture systems

Culture systems	LTC-IC frequency		Fold LTC-IC amplification
	Pre-expansion	Post-expansion
hMSCs (TPO/FL-free) culture system	1/619.57 ± 120.89	1/102 258.56 ± 9 568.49	2.79 ± 0.56
Cytokines culture system	1/625.38 ± 154.26	1/84 112.78 ± 10 867.64	3.58 ± 1.48
hMSCs coculture system	1/621.76 ± 138.59	1/93 773.42 ± 20 346.23	5.32 ± 1.73
tfhMSCs coculture system	1/617.43 ± 119.76	1/24 140.96 ± 7 586.62	10.23 ± 2.89b

^bP < 0.01 vs calculated by the Student’s t-test, when data of the tfhMSCs coculture system were compared with data in the other groups.