Assessment of liver fibrosis by a noninvasive method of transient elastography and biochemical markers

Table 1 Characteristic of the patients

Characteristics			n =30
Etiology (NBNC:B:C)	10:10:10	Choline esterase (IU/L)	262.80 ± 69.34
Background (N:CH:LC)	10:10:10	Pre-ALB (mg/dL)	21.92 ± 7.01
Sex (M:F)	22:8	RBP (mg/dL)	3.19 ± 1.39
Age (yr)	65.4 ± 10.3	LCAT (U)	100.66 ± 25.78
BMI	24.3 ± 3.1	Apo-A1 (mg/dL)	136.73 ± 32.43
ALB (mg/dL)	4.03 ± 0.60	ICGR15 (%)	12.86 ± 6.44
Total Bilirubin (mg/dL)	0.81 ± 0.28	Fibrotic area (%)	15.32 ± 9.69
PT (%)	98.83 ± 16.85	APRI	0.69 ± 0.46
PT-INR	1.01 ± 0.16	FibroScan (kPa)	15.47 ± 13.02
AST (IU/L)	41.63 ± 21.56	Hyaluronate (ng/mL)	120.50 ± 87.71
ALT (IU/L)	37.03 ± 21.58	Type IV collagen	6.85 ± 5.11
Platelet count (104/μL)	16.14 ± 4.57	HGF (ng/dL)	0.30 ± 0.09
ZTT (K-U)	13.57 ± 9.96

BMI: Body mass index; ALB: Albumin; PT: Prothrombin time; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; ZTT: Zinc turbidity test; TTT: Thymol turbidity test; GGT: Gamma glutamyl transpeptidase; APRI: AST-to platelet ratio index; HGF: Hepatocyte growth factor; RBP: Retinol-binding protein; LCAT: Lecithin-cholesterol acyltransferase; Apo: Apolipoprotein; ICG: Indocyanine.

Table 2 Correlation with fibrotic area

Markers	r	P values
ALB	-0.388	0.067
T-Bil	0.13	0.559
PT	-0.459	0.026a
PT-INR	0.472	0.022a
Platelet count	-0.58	0.003a
RBP	-0.515	0.011a
Pre-ALB	-0.609	0.002a
CholE	-0.299	0.168
LCAT	-0.447	0.032a
ApoA	-0.045	0.839
ICGR15 (%)	0.364	0.088
AST	0.295	0.173
ALT	0.243	0.266
ZTT	0.373	0.079
G-GT	0.068	0.762

r > 0.4 or r < -0.4 was considered to indicate a correlation with the fibrotic area.

^aP < 0.05 was considered to be significantly different.

Table 3 Correlation with FibroScan

Markers	r	P values
ALB	-0.446	0.013a
T-Bil	0.127	0.506
PT	-0.144	0.452
PT-INR	0.088	0.647
Platelet count	-0.431	0.017a
RBP	-0.351	0.057
Pre-ALB	-0.535	0.002a
CholE	-0.179	0.347
LCAT	-0.413	0.023a
ApoA	-0.25	0.184
ICGR15 (%)	0.45	0.012a
AST	0.21	0.268
ALT	-0.001	0.998
ZTT	0.577	0.006a
G-GT	0.118	0.538

r > 0.4 or r < -0.4 was considered to indicate a correlation with the fibrotic area.

^aP < 0.05 was considered to be significantly different.

Table 4 Comparison of diagnostic accuracy of FibroScan and other markers

Fibrotic Area >10%
	OC	Sens	Speci	SE	95% CI	AUC
FibroScan	9.1	100	76.9	0.047	0.777 - 0.999	0.932
HA	96.0	76.5	84.6	0.080	0.618 - 0.924	0.803
Col	6.6	52.9	100	0.081	0.615 - 0.923	0.801
APRI	0.57	76.5	84.6	0.081	0.615 - 0.923	0.801
Fibrotic Area > 20%
	OC	Sens	Speci	SE	95% CI	AUC
FibroScan	13.6	100	95.5	0.024	0.868 - 1.000	0.991
HA	106.0	100	77.3	0.061	0.797 - 0.993	0.946
Col	6.6	87.5	90.9	0.071	0.758 - 0.985	0.918
APRI	0.58	87.5	68.2	0.098	0.642 - 0.937	0.824

OC: Optimal cut off level; Sens: Sensitivity; Speci: Specificity; SE: Standard error; CI: Confidence interval; AUC: Area under the curve; HA: Hyaluronate; Col: Type IV collagen; APRI: AST-to platelet ratio index.