Rapid Communication
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 14, 2006; 12(26): 4241-4245
Published online Jul 14, 2006. doi: 10.3748/wjg.v12.i26.4241
Table 1 Demographic data and clinicopathological features in 38 cases of HCC
No.SexAgeTumor size(cm)TumorcapsuleMetastasisPortal vein invasionHistologicalgradeLiver cirrhosis
1M51< 5+--G2-
2M49< 5+--G3+
3M31> 10---G2+
4M595-10+--G3+
5F55> 10---G1+
6M65> 10--+G3+
7M56> 10-++G2+
8M51> 10--+G3+
9F49< 5+--G1-
10M505-10---G2+
11M53> 10--+G2+
12M43< 5+--G3+
13M365-10+--G2+
14F465-10---G1+
15F455-10---G1+
16M525-10---G2+
17F505-10---G1+
18F52< 5+--G1+
19F61> 10-++G1+
20M365-10---G2+
21M46< 5+--G2+
22M48> 10--+G2+
23F63< 5+--G1+
24M355-10+--G2-
25M74> 10-++G3+
26F555-10---G1+
27M595-10---G2+
28M30< 5+--G3+
29F565-10---G1+
30M605-10---G2+
31F65< 5+--G1+
32M59> 10-++G2+
33M61> 10-++G2+
34M55< 5---G2-
35M485-10+--G3+
36M49> 10--+G3+
37F505-10---G1+
38M58> 10-++G3+
Table 2 Primer sequences of each gene for real-time RT-PCR assay
Target geneSenseAntisense
Ang-1ACTGT GCAGA TGTAT ATCAA GCGTGGA ATCTG TCATA CTGTG AA
Ang-2GGAAG ACAAG CACAT CATCCAGTAA GCCTG ATTCC CTTCC
Tie2TCTGT GCTGT TCCTT CTTGCCTTGA GTAAC TTCCA GCGGA
VEGFAGCTA CTGCC ATCCA ATCGCGGGCG AATCC AATTC CAAGA G
G3PDHGTCAA CGGAT TTGGT CTGTA TTAGTCT TCTGG GTGGC AGTGA T
Table 3 Relation between gene expression pattern and clinicopathological parameters (mean ± SD)
Clinical parametersAng-2Ang-1Ang-2/1Tie2VEGF
Tumor size (cm)
< 5 (n = 10)0.267 ± 0.1130.148 ± 0.0892.175 ± 1.0251.686 ± 0.5500.552 ± 0.205
≥ 5 (n = 28)0.748 ± 0.4220.212 ± 0.0823.880 ± 2.0671.570 ± 0.9901.213 ± 0.537
Histlogical grade
G1 (n = 12)0.509 ± 0.3200.168 ± 0.0643.106 ± 1.4091.511 ± 0.5740.916 ± 0.497
G2 (n = 16)0.633 ± 0.3950.194 ± 0.0813.543 ± 1.9251.470 ± 0.7261.095 ± 0.604
G3 (n = 10)0.737 ± 0.5120.234 ± 0.1013.645 ± 2.4701.921 ± 0.1381.097 ± 0.585
Portal vein invasion
positive (n = 11)1.152 ± 0.3760.232 ± 0.0535.096 ± 1.6911.880 ± 1.4941.791 ± 0.423
negative (n = 27)0.405 ± 0.1630.180 ± 0.0922.753 ± 1.6311.488 ± 0.5000.732 ± 0.254
Metastasis
positive (n = 6)1.320 ± 0.3660.235 ± 0.0615.912 ± 1.8472.262 ± 1.7342.007 ± 0.214
negative (n = 32)0.490 ± 0.2710.187 ± 0.0882.967 ± 1.6001.148 ± 0.6190.857 ± 0.415
Tumor capsule
positive (n = 13)0.308 ± 0.1350.178 ± 0.1122.078 ± 0.8871.733 ± 0.4240.579 ± 0.198
negative (n = 25)0.783 ± 0.4290.203 ± 0.0684.135 ± 1.9981.529 ± 1.0561.278 ± 0.558