Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs

Table 1 Clinical data of two groups of patients

	Groups
Clinical factors	Positive anti-HBs	Negative anti-HBs	P
Age in years	40.3 ± 13.2	42.5 ± 14.7	0.7672
Sex, M/F	5/6	7/5	0.6531
Patients with HbsAg, n (%)	11 (100)	12 (100)
Patients with HbeAg, n (%)	9 (81.8)	11 (91.7)
Patients with anti-HBe, n (%)	1 (9.1)	0 (0)
ALT in IU/L	65.9 ± 30.5	98.9 ± 42.0	0.6541
HBV-DNA (log)	7.0 ± 1.60	6.87 ± 0.9	0.5263
Number of amino acid residues mutations in S gene, n (%)	34/41 (82.9)	7/41 (17.1)

Fisher’s exact test was used for the categorized data; two-tailed Student’s t test was used for ALT levels, age and HBV DNA levels (log, copies mL^-1).

Table 2 Virological and biochemical follow-up data of 8 patients

			2001				2001				2003				2004
No.	Sex	Age	ALT	HBVDNA	a-HBs	HBeAg	ALT	HBVDNA	a-HBs	HBeAg	ALT	HBVDNA	a-HBs	HBeAg	ALT	HBVDNA	a-HBs	HBeAg
1	M	57	22	0	-	-	20	0	-	-	76	8.63	-	+	87	6.38	+	+
2	M	36	48	9.38	-	+	82	8.53	-	+	21	4.04	-	+	97	3.78	+	+
3	F	65	25	4.04	+	-	58	5.04	+	-	55	6.76	+	-	55	5.08	+	-
4	M	45	45	6.86	+	+	80	6.61	+	+	59	5.57	-	+	93	6.99	+	+
5	M	55	156	7.32	+	+	116	7.11	+	+	112	7.75	+	+	149	5.80	+	+
6	F	30	19	6.91	+	+	33	7.70	+	+	20	8.86	-	+	15	9.08	+	+
8	M	38	18	0	+	-	19	0	+	-	20	0	-	-	21	4.43	+	-
10	M	72	20	5.18	+	+	25	7.04	+	+	18	6.26	-	+	48	4.15	+	+

⁺ Positive result; ^- Negative result; ALT: alanine aminotransferase; HBV DNA: HBV DNA levels (log, copies ml^-1).

Table 3 Mutations of HBV in polymerase and HBsAg protein

Mutantin patients	Position with HBsAg protein sequence change				Position with polymerase protein sequence change
(n)	145	196	198	199	173	180	204	223
Wild type	G	W	M	W	V	L	M	S
1	-	L	-	-	-	-	I	A
3	-	L	-	-	-	-	I	-
10	-	F	-	C	L	M	V	-
11	R	-	-	-	-	-	-	A
13		L	-	-	-	M	-	-