Clinicopathological and immunohistochemical analysis of gastrointestinal stromal tumor

Table 1 Primary antibody used for immunohistochemical study

Antibody	Clone	Dilution	Pretreatment
CD117	polyclonal	instant	Microwave
CD34	QBEnd/10	instant	Microwave
SMA	1A4	instant	None
Vimentin	V9	instant	None
Desmin	ZC18	instant	None
S-100	4C4.9	instant	None
NSE	E27	instant	None
NF	DA2/FNP7	instant	Microwave
MBP	polyclonal	instant	None
CgA	LK2H10	instant	Microwave
p21WAF1	4D10	1:25	Microwave
Bax	2D2	1:50	Microwave

Table 2 Relation between p21WAF1, Bax expression and clinicopathological features

Items		n	p21WAF1			Bax
			positive	%	P	positive	%	P
Localization	Stomach/	36	17	47.2	0.309	18	50	0.209
	Intestine
	Others	16	10	62.5		11	68.8
Tumor size	< 5 cm	21	12	57.1	0.535	13	61.9	0.463
	≥ 5 cm	31	15	48.4		16	51.6
Histological subtype	Spindle	20	11	55	0.720	9	45	0.086
	Epitheloid	20	9	45		10	50
	Mixed	12	7	58.3		10	83.3
Histological grade	Benign	20	1	5	0.000	2	10	0.000
	Potentially malignant	12	9	75b		11	91.7b
	Malignant	20	17	85		16	80

^bP < 0.01.

Table 3 Correlation between expressions of p21WAF1 and Bax

p21WAF1	n	Bax		r	P
		+	-
+	27	21	6	0.461	0.001
-	25	8	17