Depression of biofilm formation and antibiotic resistance by sarA disruption in Staphylococcus epidermidis

Table 1 Strains and plasmids used in the study

Strains or plasmids	Relevant characteristics	Sources or references
Strains
S. epidermidis ATCC12228	Biofilm negative	ATCC
S. epidermidis RP62A (ATCC 35984)	Biofilm positive Kanr, Eryr, Apr	ATCC
S. epidermidis RP62A△sarA	sarA deletion on the chromosome, Tetr, sarA::tet	This study
S. epidermidis RP62A (pHPS9sarA)	S. epidermidis RP62A△sarA harboring pHPS9sarA	This study
S. aureus RN4220	Restriction-negative, intermediate host for plasmid transfer from E. coli to S. epidermidis	[14]
E. coli DH5α	Φ80dlacZ△M15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK- mK+) supE44 relA1 deoR △(lacZYA-argF) U169	Promega Co.
Plasmids
pBR322	Donator of tet gene (Tetr). Apr Tetr	[15]
pBluescriptSK	E. coli cloning vector.Apr	[11]
pBT2	Temperature-sensitive shuttle vector. Apr (E. coli) Cmr (Staphylococcus)	[16]
pBT△sarA	Integration vector for homologous recombination of the△sarA gene in S. epidermidis; tet inserted into sarA locus as resistance selection marker	This study
pHPS9	Expression shuttle vector	[17]
pHPS9sarA	pHPS9 inserted sarA gene	This study

tet^r, resistance to tetracycline; kan^r, resistance to kanamycin; ery^r, resistance to erythromycin; Ap^r, resistance to ampicillin; Cm^r, resistance to chloramphenicol.