Inhibitory effects of antisense phosphorothioate oligodeoxynucleotides on pancreatic cancer cell Bxpc-3 telomerase activity and cell growth in vitro

Table 1 Absorbance values of the different groups (mean ± SD)

Groups	24 h	48 h	72 h	96 h
Control	0.48 ± 0.10	0.60 ± 0.10	0.61 ± 0.03	0.65 ± 0.05
NS-ODN (6 μmol/L)	0.50 ± 0.07	0.61 ± 0.19	0.60 ± 0.08	0.66 ± 0.19
ANS-ODN (1 μmol/L)	0.60 ± 0.03	0.48 ± 0.06	0.37 ± 0.03	0.31 ± 0.05
ANS-ODN (2 μmol/L)	0.56 ± 0.02	0.47 ± 0.09	0.34 ± 0.04	0.29 ± 0.03
ANS-ODN (4 μmol/L)	0.51 ± 0.10	0.39 ± 0.08	0.33 ± 0.04	0.28 ± 0.03
ANS-ODN (6 μmol/L)	0.43 ± 0.10	0.27 ± 0.02	0.26 ± 0.06	0.19 ± 0.00

Table 2 Ratio of inhibition of Ans-hTERT group (mean ± SD)

ANS-ODN	24 h	48 h	72 h	96 h
1 μmol/L	1.2 ± 6.4	19.6 ± 10.6	39.9 ± 4.8	52.0 ± 8.1
2 μmol/L	2.5 ± 7.5	21.1 ± 15.4	43.9 ± 6.6	55.4 ± 4.6
4 μmol/L	5.6 ± 9.7	34.6 ± 13.4	46.4 ± 6.1	57.3 ± 5.3
6 μmol/L	11.4 ± 19.2	55.6 ± 3.6	57.9 ± 9.0	71.3 ± 0.4