Caerulin-induced pancreatitis in rats: Histological and genetic expression changes from acute phase to recuperation

Table 1 Oligonucleotide primer pairs used for real-time quantitative PCR, binding site and expected size

Primer	Sequence	GenBank No.	Product size (bp)
PAPfw	5’ TGAATTATGTCAACTGGGAGAGG 3’	NM-053289.1	318
PAPrv	5’ TTACTGCTTTCCAAGACATGAGG 3’
rL13A/Fw	5’ AAGCAGGTACTGCTGGG 3’	X68282	261
rL13A/Rv	5’ CCAACACCTTGAGGCGTT 3’

Table 2 Histological changes in the pancreas of rats with caerulein-induced acute pancreatitis (mean±SE)

Period	Zymogen granule depletion	Edema	PMN infiltration	Vacuolization	Acinar disorganization
0 h	0.25 ± 0.23a,b	1.25 ± 0.31a,b	0.00 ± 0.14a	0.25 ± 0.26a	0.5 ± 0.36a
9 h	1.00 ± 0.23b,c	2.25 ± 0.31b,c	0.75 ± 0.14b	2.75 ± 0.26c	0.75 ± 0.36a,b
24 h	1.40 ± 0.20c	3.00 ± 0.28c	0.60 ± 0.12b	2.00 ± 0.24b	1.00 ± 0.32a,b
3 d	3.00 ± 0.18e	2.17 ± 0.25b,c	0.67 ± 0.11b	2.00 ± 0.22b	1.33 ± 0.29a,b
5 d	2.00 ± 0.23c,d	1.25 ± 0.31a,b	0.00 ± 0.14a	1.00 ± 0.26a,b	0.75 ± 0.36a,b
15 d	2.57 ± 0.17d,e	0.86 ± 0.23a	0.00 ± 0.10a	0.00 ± 0.20a	2.14 ± 0.28b
30 d	1.60 ± 0.20c	0.60 ± 0.83a	0.00 ± 0.12a	0.00 ± 0.24a	0.80 ± 0.33a,b
60 d	0.00 ± 0.19a	0.83 ± 0.25a	0.00 ± 0.11a	0.17 ± 0.22a	0.66 ± 0.29a
90 d	0.25 ± 0.13a,b	0.42 ± 0.18a	0.00 ± 0.07a	0.00 ± 0.15a	0.92 ± 0.21a,b

Different letters within a same column differ significantly (P < 0.05).