Basic Research
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 7, 2006; 12(25): 3999-4003
Published online Jul 7, 2006. doi: 10.3748/wjg.v12.i25.3999
Table 1 Oligonucleotide primer pairs used for real-time quantitative PCR, binding site and expected size
PrimerSequenceGenBank No.Product size (bp)
PAPfw5’ TGAATTATGTCAACTGGGAGAGG 3’NM-053289.1318
PAPrv5’ TTACTGCTTTCCAAGACATGAGG 3’
rL13A/Fw5’ AAGCAGGTACTGCTGGG 3’X68282261
rL13A/Rv5’ CCAACACCTTGAGGCGTT 3’
Table 2 Histological changes in the pancreas of rats with caerulein-induced acute pancreatitis (mean±SE)
PeriodZymogen granule depletionEdemaPMN infiltrationVacuolizationAcinar disorganization
0 h0.25 ± 0.23a,b1.25 ± 0.31a,b0.00 ± 0.14a0.25 ± 0.26a0.5 ± 0.36a
9 h1.00 ± 0.23b,c2.25 ± 0.31b,c0.75 ± 0.14b2.75 ± 0.26c0.75 ± 0.36a,b
24 h1.40 ± 0.20c3.00 ± 0.28c0.60 ± 0.12b2.00 ± 0.24b1.00 ± 0.32a,b
3 d3.00 ± 0.18e2.17 ± 0.25b,c0.67 ± 0.11b2.00 ± 0.22b1.33 ± 0.29a,b
5 d2.00 ± 0.23c,d1.25 ± 0.31a,b0.00 ± 0.14a1.00 ± 0.26a,b0.75 ± 0.36a,b
15 d2.57 ± 0.17d,e0.86 ± 0.23a0.00 ± 0.10a0.00 ± 0.20a2.14 ± 0.28b
30 d1.60 ± 0.20c0.60 ± 0.83a0.00 ± 0.12a0.00 ± 0.24a0.80 ± 0.33a,b
60 d0.00 ± 0.19a0.83 ± 0.25a0.00 ± 0.11a0.17 ± 0.22a0.66 ± 0.29a
90 d0.25 ± 0.13a,b0.42 ± 0.18a0.00 ± 0.07a0.00 ± 0.15a0.92 ± 0.21a,b