Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract

Table 1 Biopsy specimens, sex, age, and β-actin, GRP and GRP-receptor PCR amplicons detected after exposure to X–ray films for one or five days

Experimentalnumber	Tissue origin	Sex	Age	β-actin1 d	GRP1 d	GRPR
						1 d	5 d
1	Esophagus	M	64	+	+	+	+
2	Ventricle	M	64	+	-	+	+
3	Ventricle	M	84	+	+	weak	weak
4	Duodenum	M	75	+	-	-	weak
5	Duodenum	M	63	+	+	-	weak
6	Ileum	F	78	+	+	+	+
7	Caecum	M	72	+	+	+	+
8	Colon ascendens	F	68	+	-	-	weak
9	Colon ascendens	M	83	+	-	-	weak
10	Colon ascendens	F	74	+	+	-	weak
11	Colon ascendens	M	72	+	+	-	weak
12	Colon transversum	M	79	+	+	+	+
13	Colon transversum	F	79	+	+	+	+
14	Colon transversum	F	79	+	+	+	+
15	Colon transversum	F	74	+	+	-	-
16	Colon descendent	M	79	+	+	+	+
17	Colon sigmoideum	M	69	+	+	-	-
18	Colon sigmoideum	M	86	+	+	-	weak
19	Colon sigmoideum	M	48	+	+	+	+
20	Colon sigmoideum	M	83	+	+	+	+
21	Rectum	M	81	+	+	-	weak
22	Rectum	F	54	+	-	-	-
23	Rectum	M	83	+	+	+	+
24	Gallbladder	M	58	+	-	-	-
25	Pancreas	Control		+	+	+	+
26	Stomach	Contro		+	+	+	+

Table 2 PCR-primers, expected fragment sizes and PCR amplification conditions

Primer	Sequence, 5’ to 3’ orientation	Size in bp	PCR conditions
			1Cycles	Tannealing
hGRP-SE/1	AGTCTCTGCTCTTCCCAGCCTCT
hGRP-AS/1	GCAGAACTCAGTCTCTTAGGGGT	558	30	55 °C
hGRP-SE/2	CGTGCTGACCAAGATGTACC
hGRP-AS/2	TCATTGCTGGTTCAGCTGGG	349	30	62 °C
hGRPR-SE/1	AGCCCGGCATAGATCTTATCTTC
hGRPR-AS/1	AGGGGGCAAAATCAAGGGTCAAT	1477	30	55 °C
hGRPR-SE/2	CTCCCCGTGAACGATGACTGG
hGRPR-AS/2	ATCTTCATCAGGGCATGGGAG	388	30	62 °C
β-actin-SE	GCATGGAGTCCTGTCGCATCCACG
β-actin-AS	CGTCATACTCCTGCTTGCTGATCCA	2288/400	30/25	55 °C

¹Numbers of cycles in first and second round PCR amplifications;

²cDNA and gDNA PCR amplicon sizes, respectively.