Expression and activity of inducible nitric oxide synthase and endothelial nitric oxide synthase correlate with ethanol-induced liver injury

Table 1 PCR primers used for iNOS, eNOS, TNF-α and GAPDH

Name	Sense	Antisense	Product length (bp)
iNOS	TTCTTTGCTTCTGTGCTAATGCG	GTTGTTGCTGAACTTCCAATCGT	1061
eNOS	TGGGCAGCATCACCTACGATA	GGAACCACTCCTTTTGATCGAGTTAT	202
TNF-α	GCCAATGGCATGGATCTCAAAG	CAGAGCAATGACTCCAAAGT	357
GAPDH	TCCCTCAAGATTGTCAGCAA	AGATCCACAACGGATACATT	309

Table 2 Pathological scores, serum ALT levels and liver MDA contents in different experimental groups (mean±SD)

Groups	Fatty liver	Necroinflammation	ALT(U/L-1)	MDA(nmol/mg-1 protein)
4-wk dextrose	0.2 ± 0.25	1.0 ± 1.0	43.4 ± 3.05	4.83 ± 0.70
4-wk ethanol	2.1 ± 0.64a	16.1 ± 2.17 a	117.3 ± 6.92 a	8.64 ± 0.62 a
6-wk dextrose	0.6 ± 0.35	1.6 ± 1.52	46.0 ± 3.16	5.36 ± 0.31
6-wk ethanol	2.9 ± 0.64b	19.9 ± 4.29 b	124.1 ± 7.28 b	9.38 ± 0.39 b

^aP < 0.05 vs 4-wk dextrose group; ^bP < 0.05 vs 6-wk dextrose group.

Table 3 Liver NO contents, iNOS and eNOS activity in different experimental groups (mean±SD)

Groups	NO (µmol/g-1 protein)	iNOS (U/mg-1 protein)	eNOS(U/mg-1 protein)
4-wk dextrose	0.75 ± 0.14	0.27 ± 0.07	0.64 ± 0.06
4-wk ethanol	1.67 ± 0.15	0.60 ± 0.07 a	0.48 ± 0.03 a
6-wk dextrose	0.87 ± 0.07	0.35 ± 0.06	0.58 ± 0.04
6-wk ethanol	1.84 ± 0.12 b	0.70 ± 0.09 b	0.43 ± 0.05 b

^aP < 0.05 vs 4-wk dextrose group; ^bP < 0.05 vs 6-wk dextrose group.