Relationship between transforming growth factor β1 and anti-fibrotic effect of interleukin-10

Table 1 Primer sequences used in the study

mRNA	Upstreamprimer	Downstreamprimer	Productlength (bp)
TGF-β1	CTC TGC AGG CGC AGC TCT G	GGA CTC TCC ACC TGC AAG AC	392
β-actin	CCA ACC GTG AAA AGA TGA CC	CAG GAG GAG CAA TGA TCT TG	660

Table 2 Antibodies used for immunocytochemistry and immunohistochemistry

Antibody	Type	Source	Workingsolution
Desmin	Polyclonal, mouse	Beijing Zhongshan Co.	1:100
TGF-β1	Polyclonal, rabbit	BOSTER Co.	1:20/1:25
Antibody to rabbit IgG	Goat	Beijing Zhongshan Co.	Ready to use
Antibody to mouse IgG	Goat	Beijing Zhongshan Co.	Ready to use

Table 3 Expression of TGF-β1 mRNA in HSCs (mean ± SE)

Wk	n	Group N	Group C	Group I
7	5	0.143 ± 0.009	0.267 ± 0.025b	0.140 ± 0.008d
11	5	0.141 ± 0.004	0.207 ± 0.029ab	0.123 ± 0.009d

^b P < 0.01 vs group N;

^d P < 0.01 vs group C ;

^a P < 0.05 vs wk 7.

Table 4 Expression of TGF-β1 in liver tissues (mean ± SE)

GROUP	n	TGF-β1 content
		Immunhistochemistry	RT-PCR	ELISA
N2	6	0.810 ± 0.141	0.188 ± 0.014	95.442 ± 35.341
M	9	1.804 ± 0.382a	0.449 ± 0.040b	242.593 ± 53.902a
R	9	0.963 ± 0.121c	0.279 ± 0.015bd	165.514 ± 23.193ac
T	9	0.501 ± 0.250ace	0.182 ± 0.027de	113.472 ± 15.101ce