Inactivation of RASSF1A, the tumor suppressor gene at 3p21.3 in extrahepatic cholangiocarcinoma

Table 1 PCR primer sequences for RT-PCR, SSCP, MS-PCR.

Primer	Upstream sequence	Downstream sequence
RT-PCR
RT-1	5’-ACACGTGGTGCGACCTCT-3’	5’-GATGAAGCCTGTGTAAGAACCGTCCT-3’
Rt-2	5’-ACCTAGCCTTTCTCAAGCTG-3’	5’-CATCCTTGGGAGGTAAAAG-3’
SSCP
E-1a	5’-CCTGCTAGCGCCCAAAGC-3’	5’-CAGCTCCCGCAGCTCAAT-3’
E-1b	5’-ATGTCGGGGGAGCCTGAG-3’	5’-CACGGCCAGGGACCAG-3’
E-2	5’-GGGACATTTCCCCGACCT-3’	5’-ATCCTCGCCCTTCCCATAC-3’
E-3	5’-CCAGAGGCCATTTTTCAGAG-3’	5’-TAGCTGGGTACCTGCTCCTC-3’
E-4	5’-GCCGACTTCCTTTTACCT-3’	5’-GCATGCGTATATACCCTCACA-3’
E-5	5’-TGGCCCTGTCTCTGATCATT-3’	5’-TCCTCCTCCAAGCCTTACTG-3’
E-6	5’-CCTGTACACTCCCCTTTTGC-3’	5’-ACCTGGGGGTACAAGAGGTC-3’
MS-PCR
Meth-	5’-GTGTTAACGCGTTGCGTATC-3’	5’-AACCCCGCGAACTAAAAACGA-3’
Unmeth-	5’-TTTGGTTGGAGTGTGTTAATGTG-3’	5’-CAAACCCCACAAACTAAAAACAAA-3’
LOH
D3S4597	5’-CCTCACTACTCCTGGAATTG-3’	5’-CCAAGGAAGGGTTTTACTTA-3’
D3S4604	5’-ACACGTGGTGCGACCTCT- 3’	5’-GATGAAGCCTGTGTAAGAACCGTCCT-3’
D3S1568	5’-TGGCCCTGTCTCTGATCATT-3’	5’-TCCTCCTCCAAGCCTTTACTG-3’
D3S1621	5’-ACTGACATCTCACCTGGAACC-3’	5’-CACATGCAGGTGCTTGACA-3’