Effect of fermented soy milk on the intestinal bacterial ecosystem

Table 1 Composition of the anaerobic solution.

Chemicals	Weight
KH2PO4	4.5 g
Na2HPO4	6.0 g
L-cysteine HCl·H2O	0.5 g
Tween 20	1 g
Galtin	2 g
Distilled water	1 L

Table 2 Microorganisms analyzed and incubating media, times and temperatures.

Medium	Microorganism	Color of the colony	Incubation conditions
Bifidobacteria iodoacetate medium25 (BIM-25)	Bifidobacterium spp.	Green	48 h, 35-37 °C
Lactobacillus anaerobic MRS with bromocresol green (MRS-modified)	Lactobacillus spp.	Reddish orange	48 h, 35-37 °C
Tryptose-sulfite-D-cycloserine agar (TSC)	Clostridium perfringens	White with black center	24 h, 35-37 °C
Endo agar plates (Endo)	Coliform organisms	Metallic red	24 h, 35-37 °C
CDC anaerobe blood agar plates (CDC)	Total anaerobic organisms	White	48 h, 35-37 °C

Table 3 Background information of subjects before and after the study.

Group	n	Age (yr)	Wk 0			Wk 8
			Weight (kg)	Height (cm)	BMI	Weight (kg)	Height (cm)	BMI
A	14	23.1±1.1	56.4±10.6	162.0±7.9	21.3±2.3	56.1±10.2	162.0±7.9	21.2±2.2
B	14	22.6±1.8	55.9±8.3	162.6±8.2	21.1±1.5	56.1±9.5	163.1±8.8	21.1±3.5