Pharmacokinetics of C-1027 in mice as determined by TCA-RA method

Table 1 Precision and accuracy for determination of ¹²⁵I-C-1027 by TCA-RA method in mouse serum (mean±SD, n = 5).

Added (ng/mL)	Within-day			Between-day
	Found	RSD (%)	Accuracy (%)	Found	RSD (%)	Accuracy (%)
1	0.98±0.05	5	98	0.96±0.04	4.6	96
2.5	2.47±0.05	1.9	98.6	2.45±0.25	10.1	98.2
20	9.81±0.14	0.7	99	19.65±1.00	5.1	98.4

Table 2 Mean pharmacokinetic parameters of ¹²⁵I-C-1027 measured by TCA-RA method at three doses in mice (n = 6).

Parameters	Unit	Doses (mg/kg)			P
		10	50	100
A	ng/mL	34.62	142.98	277.02
a	/h	9.61	9.64	4.92
B	ng/mL	3.31	18.1	26.02
b	/h	0.22	0.2	0.18
V(d)	L/kg	0.26	0.31	0.33	>0.05
T1/2a	h	0.07	0.07	0.14
T1/2b	h	3.1	3.4	3.9	>0.05
K21	/h	1.04	1.26	0.58
K10	/h	2.06	1.55	1.49	>0.05
K12	/h	6.73	7.03	3.02
AUC	ng.h/mL	18.41	103.69	202.74
CL(S)	L/(kg.h)	0.54	0.48	0.49	>0.05

V_(d): Apparent distribution volume; T_1/2: Half-life; K: Elimination rate constant; AUC: Area under curve; CL_(S): Clearance.