Selection of optimal antisense accessible sites of survivin and its application in treatment of gastric cancer

Table 1 Antisense accessible regions of survivin mRNA shown by primer extension analysis.

Primer name	Product size of primerextension (bp)	3’ end site of primer extension (bp)	3’ end site of RNase Hcleavage (bp)	Antisense accessiblesites (bp)
P1	42	255	254	235-254
	60	237	236	217-236
	70	227	226	207-226
	82	215	214	195-214
	90	207	206	187-206
	104	193	192	173-192
	118	179	178	159-178
	151	146	145	126-145
P2	40	124	123	104-123
	70	94	93	74-93
	100	64	63	44-63
	118	46	45	26-45
	140	24	23	4-23

Table 2 Antisense accessible sites and their complementary oligonucleotide sequences selected by random oligonucleotide library/RNase H cleavage combined with RNADraw analysis.

Serial number	Base sequence	Origination-end sites	Base pair
ODN1	AGATGACGACCCCATAGAGG	207-226	20 bp
AS-ODN1	CCTCTATGGGGTCGTCATCT
ODN2	GAGCTGGAAGGCTGGGAGCC	187-206	20 bp
AS-ODN2	GGCTCCCAGCCTTCCAGCTC
ODN3	CTTCATCCACTGCCCCACTG	126-145	20 bp
AS-ODN3	CAGTGGGGCAGTGGATGAAG
ODN4	AGGACCACCGCATCTCTACA	44-63	20 bp
AS-ODN4	TGTAGAGATGCGGTGGTCCT