Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas

doi:10.3748/wjg.v11.i5.629

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Feb 7, 2005 (publication date) through Nov 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Feb 7, 2005; 11(5): 629-633
Published online Feb 7, 2005. doi: 10.3748/wjg.v11.i5.629

Table 1 Sequences and coordinates of primers and probes for RT-PCR analysis.

Transcript	Oligonucleotide sequence (5’-3’)	Product size (bp)	Genome coordinate
Wp	5’primer CAGGAGATCTGGAGTCCACACAAATCCT	131/136	14396-14556
	5’primer GAGGAGATCTGGAGTCCACACAAATGGG		14396-14561
	3’primer ACTGAAGCTTGACCGGTGCCTTCTTAGGAG		14735-14716
	probe GAGACCGAAGTGAAGGCCCTGGACCAACCC		14561-14590
Cp	5’primer TGTAGATCTGATGGCATAGAGAC	285/290	11342-11355
	3’primer ACTGAAGCTTGACCGGTGCCTTCTTAGGAG		14735-14716
	probe AAGGACACCGAAGACCCCCAGAG		11356-11378
Qp	5’primer ATATGAGCTCGTGCGCTACCGGATGGCG	255	62441-62457
	3’primer GATCGAATTCCATTTCCAGGTCCTGTACCT		107987-107967
	probe GGTGAATCTGCTCCCAGGTC		67628-67609
EBNA1	5’primer GATGAGCGTTTGGGAGAGCTGATTCTGCA	273	67510-67539
	3’primer TCCTCGTCCATGGTTATCAC		108075-108056
	probe AGACCTGGGAGCAGATTCAC		67608-67627
EBNA2	5’primer GCTGCTACGCATTAGAGACC	339	47892-47911
	3’primer TCCTGGTAGGGATTCGAGGG		48616-48597
	probe CAGCACTGGCGTGTGACGTGGTGTAAGTT		48391-48420
LMP1	5’primer TCCTCCTCTTGGCGCTACTG	490	169383-169364
	3’primer TCATCACTGTGTCGTTGTCC		168740-168759
	probe GAACAGCACAATTCCAAGGAACAATGCCTG		169061-169090
LMP2A	5’primer ATGACTCATCTCAACACATA	280	166874-166893
	3’primer CATGTTAGGCAAATTGCAA		380-361
	probe ATCCAGTATGCCTGCCTGTA		62-81
LMP2B	5’primer CAGTGTAATCTGCACAAAGA	325	169819-169838
	3’primer CATGTTAGGCAAATTGCAAA		380-361
	probe ATCCAGTATGCCTGCCTGTA		62-81
BZLF1	5’primer ATTGCACCTTGCCGCCACCTTTG	608	103194-103180
	3’primer CGGCATTTTCTGGAAGCCACCCGA		102486-102463
	probe CACTGCTGCTGCTGTTTGAACAGT		102772-102795
BRLF1	5’primer ACCATACAGGACACAACACCTC	266	106166-106145
	3’primer GATGTTGAGCGTGGCCATTAGC		104959-104980
	probe GTTAGCCTCAGAAAGTCTTCCAAGCCATCC		105140-105169
BARF1	5’primer GGCTGTCACCGCTTTCTTGG	203	165560-165579
	3’primer AGGTGTTGGCACTTCTGTGG		165762-165743
	probe CTGGTTTAAACTGGGCCCAGGAGAGGAGCA		165644-165673
BHRF1	5’primer GTCAAGGTTTCGTCTGTGTG	211	53830-53849
	3’primer TTCTCTTGCTGCTAGCTCCA		54480-54461
	probe ATGCACACGACTGTCCCGTATACAC		54435-54411
BcLF1	5’primer TGCCCAATCCCAAGTACACGACC	377	136229-136207
	3’primer CAGCAGGTCATAATTGGACGGG		135853-135874
	probe GAGAGCATTCTGTAGGTTAAACGCGAGGA		136099-136128
BLLF1	5’primer CCTACCTTGAATACAACTGG	309	90860-90841
	3’primer TGACGCTTGGCTGGTGGTGC		89961-89980
	probe TGGTGACATCCGCGGTGGAT		90731-90750

Citation: Luo B, Wang Y, Wang XF, Liang H, Yan LP, Huang BH, Zhao P. Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas. World J Gastroenterol 2005; 11(5): 629-633
URL: https://www.wjgnet.com/1007-9327/full/v11/i5/629.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i5.629