Haplotype of prostaglandin synthase 2/cyclooxygenase 2 is involved in the susceptibility to inflammatory bowel disease

Table 1 Genotype frequencies and odds ratios for UC and CD of PTGS2 SNPs

Genotype	Controls		UC			CD
	n1	%	n1	%	OR(95%CI)2	n1	%	OR(95%CI)2
PTGS2.401
A/A	277	79	115	82	1	107	77	1.00
A/G	66	19	24	17	0.82 (0.48 – 1.39)3	30	22	1.17 (0.70 –1.94)3
G/G	7	2	1	1		1	1
Total	350	100	140	100		138	100
PTGS2.926
G/G	256	75	112	81	1	106	76	1.00
G/C	75	22	23	17	0.77 (0.47 – 1.28)3	31	22	1.06 (0.65 –1.73)3
C/C	8	2	4	3		3	2
Total	339	100	139	100		140	100
PTGS2.3050
G/G	250	71	109	75	1	102	71	1
G/C	99	28	32	22	0.83 (0.52 – 1.32)3	38	26	0.97 (0.62 –1.53)3
C/C	1	1	4	3		4	3
Total	350	100	145	100		144	100
PTGS2.5209
T/T	265	75	110	76	1	95	66	1
T/G	81	23	30	21	1.00 (0.62 - 1.61)3	46	33	1.63 (1.03 - 2.57)3
G/G	7	2	5	3		2	1
Total	353	100	145	100		143	100
PTGS2.8473
C/C	44	13	18	14	1.003	21	15	1.003
T/C	147	42	49	34		54	38
T/T	155	45	78	54	1.50 (1.00 - 2.27)	68	47	1.10 (0.72 - 1.68)
Total	346	100	145	100		143	100

¹Numbers may not sum up to the totals of controls or cases due to genotyping failure. All samples that did not give a reliable result in the first round of genotyping were resubmitted to up to three additional rounds of genotyping. Data points that were still not filled after this procedure were left blank.

²All ORs are adjusted for sex and age.

³Heterozygotes and homozygotes for the rare allele have been grouped in order to improve statistical power

Table 2 PTGS2 haplotype frequencies and odds ratios for IBD

Haplotype1	Controls	UC cases		CD cases		UC+CD cases2
	n (%)	n (%)	OR(95% CI)	n (%)	OR (95% CI)	n (%)	OR (95% CI)3
AGTT	471 (64.7)	193 (65.6)	1.00 (Ref.)	183 (62.7)	1.00 (Ref.)	376 (64.2)	1.00 (Ref.)
AGTC	147 (20.2)	55 (18.7)	0.91 (0.64 - 1.30)	54 (18.5)	0.95 (0.66 - 1.35)	109 (18.6)	0.93 (0.69 - 1.24)
GCGC	64 (8.8)	22 (7.5)	0.84 (0.50 - 1.39)	27 (9.2)	1.09 (0.67 - 1.75)	49 (8.4)	0.96 (0.63 - 1.45)
ACGC	25 (3.4)	8 (2.7)	0.78 (0.35 - 1.73)	7 (2.4)	0.72 (0.31 - 1.66)	15 (2.6)	0.75 (0.36 - 1.51)
AGGT	2 (0.3)	10 (3.4)	12.2 (2.97 – inf.)	9 (3.1)	11.58 (2.79 -inf.)	19 (3.2)	11.9 (2.83 – 105.76)
Rare4	19 (2.6)	6 (2.0)	0.77 (0.31 - 1.90)	12 (4.1)	1.63 (0.78 - 3.37)	18 (3.1)	1.19 (0.61 – 2.29)

¹The order of SNPs in the haplotypes is PTGS2.401, PTGS2.926, PTGS2.5209, PTGS2.8473 .

²UC and CD combined.

³ORs are not corrected, they are based on population level haplotype frequencies and are relative to the most common haplotype (AGTT).

⁴Rare haplotypes (frequency ≤1% in both cases and controls)