Separation of growth-stimulating peptides for Bifidobacterium from soybean conglycinin

Table 1 Effect of soybean conglycinin hydrolysates on the growth of bifidobacterium longum (n = 9)

Group	Control	Conglycinin	PTC	HCl-FHC
A460	0.11 ± 0 .04	0.31 ± 0 .11	0.66 ± 0 .07bd	0.12 ± 0 .02

Values are expressed as the mean±SD.

^bP<0.01 vs control group,

^dP<0.01 vs HCl-FHC group.

Table 2 Growth-stimulating activity of the isolated fractions from pepsin-treated conglycinin on bifidobacteria (n = 9)

	Control	P1	P2	P3	P4	P5	PTC	Conglycinin	HCl-FHC
A460 nm	0.35 ± 0 .05	0.38 ± 0 .05	0.50 ± 0 .10a	0.45 ± 0 .09a	0.33 ± 0 .08	0.36 ± 0 .07	0.42 ± 0 .06	0.37 ± 0 .05	0.36 ± 0 .08

Values are expressed as the mean±SD,

^aP<0.05 vs control group.

Table 3 Effect of conglycinin peptides on levels of serum IL-2 and small intestinal sIgA in mice (n = 8)

Group	Control	Conglycinin	PTC	P2-PTC	HCl-FHC
sIgA (ng/g)	118.27 ± 33.93	133.17 ± 36.15	139.01 ± 32.65	172.08 ± 35.40ab	104.43 ± 30.75
IL-2 (ng/mL)	0.602 ± 0.255	0.445 ± 0.112	0.602 ± 0.351	0.693 ± 0.305	0.618 ± 0.141

Values are expressed as the mean±SD,

^aP<0.05 vs control group,

^bP<0.01 vs HCl-FHC group.

Table 4 F-6-PPK activities in cecal contents (n = 8)

Group	Control	Conglycinin	PTC	P2-PTC	HCl-FHC
A 505 nm	0.19 ± 0.03	0.36 ± 0.06ac	0.56 ± 0.08bd	0.44 ± 0.12bd	0.22 ± 0.10

Values are expressed as mean±SD,

^aP<0.05,

^bP<0.01 vs control group,

^cP<0.05,

^dP<0.01 vs HCl-FHC group.

Table 5 Effect of conglycinin peptides on pH and total number of enterococci and E.coli of cecum in mice (n = 8)

Group	Control	Conglycinin	PTC	P2-PTC	HCl-FHC
pH	7.21 ± 0.09	7.19 ± 0.06	7.17 ± 0.09	7.08 ± 0.08a	7.18 ± 0.09
E.coli log10CFU/g	6.32 ± 0.34	6.21 ± 0.5	6.19 ± 0.16	6.19 ± 0.16	6.27 ± 0.30
Enterococcilog10CFU/g	5.78 ± 0.19	5.55 ± 0.29	5.42 ± 0.37	5.38 ± 0.26a	5.66 ± 0.14

Values are expressed as the mean±SD,

^aP<0.05 vs control group.