Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

Table 1 HBV status in sera of HBV carriers

Sample	Antigen-antibody test1					ALT2	Bilirubin
	HBsAg	HBsAb	HBeAg	HBeAb	HBcAb
1	+	–	+	–	+	39.8	11.7
2	+	–	+	–	+	18.7	7.5
3	–	+	–	–	–	71.7	19
4	–	+	–	–	–	33.8	4.6
5	–	–	–	–	–	18.7	6.2

¹HBsAg: hepatitis B surface antigen; HBsAb: antibody to HBsAg; HBeAg: hepatitis B e antigen; anti-HBe: antibody to HBeAg; anti-HBc: antibody to hepatitis B core antigen (HBcAg).

²ALT: alanine aminotransferase. +Represents positive; – represents negative.

Table 2 Viral load in sera and semen of HBV carriers

Sample1	Method of HBV DNA Isolation	HBV DNA (copies/mL)2			Mean	SD3
		1	2	3
Sera-1	Qiagen kit	6.73 × 106	7.14 × 106	8.8 × 106	7.56 × 107	1.11 × 107
Semen-1	Qiagen kit	2.18 × 105	2.34 × 105	1.69 × 105	2.14 × 105	4.31 × 104
Semen-1	Phenol	1.82 × 105	2.31 × 105	1.98 × 105	2.04 × 105	2.52 × 104
Sera-2	Qiagen kit	1.82 × 107	1.73 × 107	1.47 × 107	1.67 × 107	1.82 × 106
Semen-2	Qiagen kit	2.81 × 105	2.59 × 105	3.67 × 105	3.02 × 105	5.71 × 104
Semen-2	Phenol	3.58 × 105	2.62 × 105	2.41 × 105	2.87 × 105	5.22 × 105

¹Sample from patient 1 or 2.

²Data show the average of each independent experiment.

³SD represents standard deviation.

Table 3 Viral load in different volumes of semen (copies/mL)

Sample	Method of HBVDNA isolation	10 μL	50 μL	200 μL
Semen-1	Qiagen kit	2.37 × 105	2.26 × 105	2.43 × 105
	Convention	2.25 × 105	3.04 × 105	1.80 × 105
Semen-2	Qiagen kit	2.76 × 105	2.92 × 105	2.87 × 105
	Convention	2.38 × 105	2.56 × 105	2.74 × 105