Persistence of gene expression changes in noninflamed and inflamed colonic mucosa in ulcerative colitis and their presence in colonic carcinoma

Table 1 Primer sets for reverse transcription-PCR

Gene	5’-Forward	3’-Reverse
Homeo box a4	ATGACCATGAGCTCGTTTTTGAT	TATGGAGGAGGGAACGGGTG
Mads box transcription factor 2b	ATGGGGAGGAAAAAAATCCAGA	CTGTTGGGTCTTCTCTGAAGA
G3PDH	ACCACAGTCCATGCCATCAC	TCCACCACCCTGTTGCTGTA

Table 2 Gene transcripts displaying a two fold or higher increase or decrease in expression level that was significant at the P<0.05 level

Accession number	Gene name	AI in controls	AI in UC	Control/UC	P
NM_002141	Homeo box a4 (Hoxa4)	356 193.4	144 705.0	2.46	0.005
NM_005919	Mads box transcription enhancer factor 2, polypeptide b (MEF2b)	283 291.9	113 174.8	2.5	0.009
XM_001566	Calcium activated chloride channel 3 precursor (CLCA3)	5 308.8	1 188.9	4.46	0.01
NM_032946	Nuclear rna export factor 5 (NXF5)	4 416.7	1 681.3	2.63	0.014
NM_022061	Ribosomal protein l17 isolog (RPL7)	299 317.9	143 755.9	2.08	0.027
NM_016638	Srp25 nuclear protein (SRP25)	2 872.1	6 576.4	0.43	0.043

AI: average intensity.