Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat

Table 1 Primer sequences used in this study

mRNA	Sense primers	Antisense primers	Product length (bp)
TGF-β1	CTC TGC AGG CGC AGC TCT G	GGA CTC TCC ACC TGC AAG AC	392
EGF	GAC AAC TCC CCT AAG GCT TA	CATGCA CAC GCC ACC ATT	567
GAG		GCA GTA CCC ATC GTA CGA
HGF	TCT TGA CCC TGA CAC CCC	GTG ATT CAG CCC CAT CCG G	269
PDGF	GAT CCG CTC CTT TGA TGA TC	GTC TCA CAC TTG CAT GCC AG	435
β-actin1	AGC ACT TGC GGT CCA CGA	GAG CTA TGA GCT GCC TGA	410
β-actin2	CCA ACC GTG AAA AGA TGA CC	CAG GAG GAG CAA TGA TCT TG	660

Table 2 Antibodies used for immunocytochemistry

Antibody	Type	Source	Working solution
Desmin	Polyclonal, mouse	Beijing Zhongshan Co.	0.111111111
TGF-β1	Polyclonal, rabbit	BOSTER Co.	1:20
EGF	Polyclonal, rabbit	BOSTER Co.	1:50
PDGF	Polyclonal, rabbit	Beijing Zhongshan Co.	1:20
Antibody to rabbit IgG	Goat	Beijing Zhongshan Co.	Ready to use
Antibody to mouse IgG	Goat	Beijing Zhongshan Co.	Ready to use

Table 3 Expression of TGF-β1 mRNA in HSCs (mean±SE)

Wk	n	Group N	Group C	Group I
7th wk	5	0.143±0.009	0.267±0.025b	0.140±0.008d
11th wk	5	0.141±0.004	0.207±0.029ab	0.123±0.009d

^aP<0.05 vs 7^th wk;

^bP<0.01 vs group N;

^dP<0.01 vs group C.

Table 4 Expression of EGF mRNA in HSCs (mean±SE)

Wk	n	Group N	Group C	Group I
7th wk	5	0.181±0.012	0.425±0.013b	0.293±0.024b,d
11th wk	5	0.179±0.004	0.514±0.008a,b	0.258±0.021c,d

^aP<0.05 vs 7^th wk;

^bP<0.01 vs group N;

^cP<0.05 vs group N;

^dP<0.01 vs group C.

Table 5 Expression of HGF mRNA in HSCs (mean±SE)

Wk	n	Group N	Group C	Group I
7th	5	0.364±0.015	0.636±0.009b	0.210±0.010bd
11th	5	0.383±0.004	0.622±0.022b	0.265±0.028ad

^aP<0.05 vs group N;

^bP<0.01 vs group N;

^dP<0.01 vs group C.