Gastrin and antral G cells in course of Helicobacter pylori eradication: Six months follow up study

Table 1 Clinic and demographic data in H pylori positive patients with DU and gastritis and in the control groups at the beginning of the study

	H pylori +Ge (n = 24)	H pylori+ Gne (n = 7)	H pylori +DU (n = 8)	CG1 (n = 11)	CG2 (n = 7)	P
Age (yr)	47 ± 13	48 ± 9	47 ± 23	47 ± 19	33 ± 11	NS
Sex (males)	13	1	2	5	2	NS
Smokers	8	3	2	4	4	NS
Alcohol intake	12	3	1	1	1	NS
PH of PUD	8	0	6	7	0	NS
FH of PUD	11	3	5	6	3	NS

H pylori +Ge-H pylori+eradicated patients with gastritis; H pylori+Gne-H pylori+non eradicated patients with gastritis; H pylori +DU- patients with H pylori+duodenal ulcer; CG1-H pylori negative dyspeptic patients; CG2- asymptomatic volunteers; PUD -peptic ulcer disease; PH-personal history; FH- family history; NS-not statistically significant.

Table 2 Antral G cell in dyspeptic patients before and after eradication therapy-morphometric analysis on light microscopy

	H pylori +Ge	H pylori +Gne	H pylori +DU	CG1	CG2	P
	(n = 24)	(n = 7)	(n = 8)	(n = 11)	(n = 7)
G cell number (per mm2 of antral mucosa)
Before therapy	416 ± 40	405 ± 32	292 ± 20a	413 ± 61	426 ± 57	0.05a
After therapy	294 ± 32a	432 ± 18	400 ± 32b	428 ± 80	/	NS
P	0.05a	NS	0.01b	NS	/
G cell volume density (%)
Before therapy	0.48 ± 0.09	0.43 ± 0.11	0.42 ± 0.02	0.48 ± 0.03	0.44 ± 0.02	NS
After therapy	0.31 ± 0.02a	0.40 ± 0.04	0.43 ± 0.01	0.50 ± 0.03	/	NS
P	0.05	NS	NS	NS	/
Antral G/ total antral endocrine cells ratio
Before therapy	0.44 ± 0.04	0.40 ± 0.03	0.37 ± 0.05	0.44 ± 0.29	0.45 ± 0.07	NS
After therapy	0.44 ± 0.02	0.47 ± 0.07	0.41 ± 0.07	0.44 ± 0.37	/	NS
P	NS	NS	NS	NS	/

H pylori+Ge- H pylori+eradicated patients with gastritis; H pylori+Gne-H pylori+non eradicated patients with gastritis; H pylori+DU-patients with H pylori+duodenal ulcer; CG1-H pylori negative dyspeptic patients; CG2- asymptomatic volunteers; NS- P>0.05;

^aP<0.05;

^bP<0.01.

Table 3 Ultrastructural characteristics of antral G cell in dyspeptic patients before and after therapy assessed by electron microscopy morphometric study

Antral G cell	H pylori+Ge	H pylori+Gne	H pylori+DU	CG1	CG2	P
	(n = 24)	(n = 7)	(n = 8)	(n = 11)	(n = 7)
Number of cells analyzed
Before therapy	93	30	33	55	37
After therapy	113	31	30	47
Cell profile surface (μm2)
Before therapy	142 ± 14	138 ± 20	188 ± 14a	130 ± 10	136 ± 10	0.05a
After therapy	140 ± 15	133 ± 12	138 ± 10d	133 ± 12	/	NS
P	NS	NS	0.001d	NS	/
Endoplasmatic reticulum (μm2) (profile surface)
Before therapy	6.9 ± 2.3a	6.2 ± 2.3	17.9 ± 2.3b	5.3 ± 0.9	5.5 ± 0.9	a0.05;b0.01
After therapy	5.0 ± 2.3	6.0 ± 1.0	5.7 ± 1.3d	5.5 ± 0.7		NS
P	NS	NS	0.001d	NS
Volumen density of cytoplasmic granules
Before therapy	17.3 ± 1.9a	13.1 ± 3.2	47.0 ± 4.9b	14.6 ± 2.7	13.6 ± 2.7	a0.05;b0.01
After therapy	9.7 ± 1.1b	14.2 ± 3.1	17.3 ± 1.9	13.2 ± 2.0	/	NS
P	0.01b	NS	NS	NS	/
Number of cytoplasmic granules/cell profile
Before therapy	232 ± 18a	199 ± 34	330 ± 13b	190 ± 37	196 ± 21	a0.05;b0.01
After therapy	180 ± 13	202 ± 45	220 ± 31	196 ± 20	/	NS
P	NS	NS	NS	NS	/
Mean diameter of cytoplasmic granules (nm)
Before therapy	220 ± 23	245 ± 11	327 ± 23a	237 ± 4	227 ± 7	0.01a
After therapy	223 ± 23	229 ± 22	257 ± 21b	223 ± 9	/	NS
P	NS	NS	0.01b	NS	/
Golgi apparatus (μm2) (profile surface)
Before therapy	2.9 ± 0.10b	2.10 ± 0.3	3.33 ± 0.10a	2.11 ± 0.33	2.05 ± 0.23	0.01a
After therapy	2.15 ± 0.10	2.23 ± 0.2	2.20 ± 0.11b	2.20 ± 0.22	/	NS
P	0.01b	NS	0.01b	NS	/

^aP<0.05,

^bP<0.01,

^dP<0.001 vs others.