Comparative proteome analysis of untreated and Helicobacter pylori-treated HepG2

Table 1 Matching of protein spot-10 peptide mass fingerprint data with protein Q96AX2 in database.

User mass	Matching mass	Mass (Da)	#MC	Modification	Position	Peptide
976.901	977.3638	0.4628	1	1xCys_CAM	215-222	RSSCCSFM
990.94	991.3795	0.4395	1	1xCys_PAM	215-222	RSSCCSFM
999.094	999.5332	0.4392	0		42-49	TCFLIQFK
1006.907	1007.3744	0.4674	1	1xCys_PAM,1xMSO	215-222	RSSCCSFM
1167.021	1166.5295	-0.4914	0	2xCys_CAM,1xGERA,1xMSO	216-222	SSCCSFM
1266.017	1265.6091	-0.4078	1	1xCys_CAM,1xGERA,1xMSO	215-222	RSSCCSFM
1308.278	1308.742	0.464	0	2xGERA	216-222	SSCCSFM
1345.559	1345.707	0.148	1		151-162	VIRSEDGETLAR
1464.517	1464.8431	0.3261	1	2xGERA	215-222	RSSCCSFM
1466.362	1466.8112	0.4492	0	2xCys_PAM,2xGERA,1xMSO	216-222	SSCCSFM
1516.378	1516.7543	0.3763	0		122-133	AWLTEIHEYAQR
1684.665	1684.8249	0.1599	1		1-17	TGTPGAVATRD GEAPER
2479.013	2479.2207	0.2077	1	1xCys_CAM	18-41	SPPCSPSYDLTGK VMLLGDTGVGK

Table 2 Proteins identified by MS.

Spot	SWISS-PROT Accession No.	Peptides Matched	pI	Mr (Da)	Sequence coverage (%)	Protein identified
1	P05556	19/106	5.31	86242	31.2	Chain 1: integrin β-1
3	P17252	7/28	6.61	76764	39.9	Protein kinase C, alpha type
6	P48742	6/34	7.16	44482	39.6	LIM/homeobox protein Lhx1
7	P20042	10/92	5.60	38388	30.9	eIF-2-beta
8	P46734-3	6/37	7.08	39940	35.0	MAP kinase kinase 3
9	P48059	8/44	8.43	37251	36.3	PINCH protein
10	Q96AX2	13/86	5.96	24684	36.5	Ras-related protein Rab-37