Intravenous acid fibroblast growth factor protects intestinal mucosal cells against ischemia-reperfusion injury via regulating Bcl-2/Bax expression

Table 1 Oligonucleotide primers for RT-PCR detection of bax, bcl-2 and β-actin mRNA transcripts.

Genes	Upper primers	Lower primers
bax	5’-AGGTCTTTTTCCGAGTGGCAGC-3’	5’-CCCGGAGGAAGTCCAATGTCC-3’
bcl-2	5’-GACTTCGCCGAGATGTCCAG-3’	5’-GTGCAGGTGCCGGTTCAGG-3’
β-actin	5’-AGCCATGTACGTAGCCATCC-3’	5’-GCCATCTCTTGCTCGAAGTC-3’

Table 2 Effect of aFGF on the apoptotic rates in intestinal mucosa after ischemia-reperfusion insult (n = 6, mean±SD, %).

Groups	Group R	Group A
Group C	19.67±3.50	19.67±3.50
Group I	27.67±9.63	27.67±9.63
15 min after reperfusion	29.50±5.61	25.17±6.43
30 min after reperfusion	31.00±5.02a	26.00±4.86
1 h after reperfusion	34.67±5.47a	32.83±7.08a
2 h after reperfusion	50.67±6.95a	41.17±3.49ac
6 h after reperfusion	54.17±7.86a	42.83±5.23ac
12 h after reperfusion	64.33±6.47a	53.33±6.92ac
24h after reperfusion	28.50±5.47	23.33±3.83
48 h after reperfusion	26.00±5.76	22.00±4.60

^aP<0.05 vs group C;

^cP<0.05 vs group R at matched time points.

Table 3 Effect of aFGF on protein contents of bax and bcl-2 in intestinal villus after ischemia-reperfusion (n = 6, mean±SD, %).

Groups	Bax protein		Bcl-2 protein
	Group R	Group A	Group R	Group A
Group C	19.83±3.66	19.83±3.66	35.33±4.59	35.33±4.59
Group I	23.83±7.65	23.83±7.65	28.17±4.12	28.17±4.12
15 min after reperfusion	25.00±5.93	19.83±5.27	29.83±6.12	31.17±5.04
30 min after reperfusion	28.00±9.23	20.33±4.76	28.83±5.85	30.17±5.95
1 h after reperfusion	37.83±4.75a	24.17±4.49c	24.50±6.16a	26.00±5.10a
2 h after reperfusion	47.67±7.26a	37.50±7.01ac	20.67±7.15a	29.67±4.84c
6 h after reperfusion	54.67±5.28a	47.00±3.52ac	18.83±5.35a	27.83±2.72c
12 h after reperfusion	46.33±4.27a	37.67±3.83ac	19.50±4.37a	25.67±4.03ac
24 h after reperfusion	29.00±4.43	25.00±4.34	28.67±2.94	29.33±6.02
48 h after reperfusion	26.17±3.49	22.83±3.19	29.00±3.74	30.50±4.89

^aP<0.05 vs group C;

^cP<0.05 vs group R at matched time points.

Table 4 Effect of aFGF on gene expression of bax and bcl-2 in intestinal villus after ischemia-reperfusion (n = 6, mean±SD, %).

Groups	Bax gene		bcl-2 gene
	Group R	Group A	Group R	Group A
Group C	80.956±8.141	85.729±7.523	24.614±3.175	27.803±3.347
Group I	105.961±8.880a	97.815±9.568a	11.221±2.752a	10.744±3.549a
15 min after Reperfusion	93.937±3.281a	83.928±6.659c	23.057±4.642	20.457±5.013a
30 min after Reperfusion	94.138±4.583a	94.288±3.707	12.921±3.059a	17.961±1.653ac
1 h after Reperfusion	108.367±12.668a	76.063±6.337c	11.789±3.200a	20.529±2.200ac
2 h after Reperfusion	90.176±6.630	76.723±6.153c	9.083±2.168a	20.655±1.544ac
6 h after Reperfusion	85.837±6.037	62.415±3.535ac	6.512±2.477a	15.510±1.757ac
12 h after Reperfusion	86.987±6.331	80.155±6.756	7.882±2.086a	27.349±3.592c
24 h after Reperfusion	85.230±9.132	90.479±5.137	15.898±2.393a	30.364±2.298c
48 h after Reperfusion	86.728±7.278	94.846±14.403	14.755±3.268a	23.937±2.726c

^aP<0.05 vs group C;

^cP<0.05 vs group R at matched time points.