Mutation in D-loop region of mitochondrial DNA in gastric cancer and its significance

Table 1 Primers for amplification and their nucleotide sequences.

Primers		Nucleotide sequence
Upper	nt15791-15810	5'-ATCATTGGACAAGTAGCATC-3’
Down	nt725-706	5'-GGTGAACTCACTGGAACGGG-3’

Table 2 Primers for sequencing and their nucleotide sequences.

Primers		Nucleotide sequence
Upper	nt111-nt130	5'-ACCCTATGTCGCAGTATCTG-3’
Upper	nt16328-16347	5'-CGTACATAGCACATTACAGT-3’
Down	nt16540-16514	5'-GTGGGCTATTTAGGCTTTATGACCCTG-3’

Table 3 Mutations in D-loop region of mitochondrial DNA in gastric cancer.

Location	Cambridge sequence	Cancer nucleotide	Normal tissue nucleotide
16122	T	C	T
16221	C	T	C
16234	C	Deletion	C
16366	T	C	T
16401	C	A	C
16438	G	A	G
16465	A	G	A
16492	A	Deletion	A
16494	C	T	C
41	C	T	C
72	T	C	T
167	C	G	C
255	G	A	G
303	(C)7	(C)8	(C)7
303	(C)7	(C)9	(C)8
392	T	C	T
514	(CA)5	(CA)6	(CA)5
567	(C)6	(C)7	(C)6

Table 4 ROS level and cell cycle in mutation group and control group (mean±SD).

	ROS (MFI)	Apoptosis (%)	G0/G1 (%)	S (%)	G2/M (%)
Mutation group n = 7	156.3±9.7a	12.5±1.5a	57.3±5.3	22.3±2.4a	20.6±2.1
Control group n = 13	117.3±10.4	8.6±0.1	65.4±5.6	16.4±1.5	18.1±1.3

^aP<0.05 vs control group.