Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma

Table 1 Specification of primer sets for polymerase chain reaction analysis.

Primer	Sequence	PCR product (bp)
IGF-1	5-CAACAAGCCCACAGGGTATGGC-3’
	3-GGAAACGAGACGTGCTCAATGGACA-5’	312
IGF-1R	5-ATGCTGTTTGAACTGATGCGCA-3’
	3-GCCCCCGGCGTTCTTGCTCGCC-5’	354
Gastrin	5-GTCTATGTGCTGATCTTTGCACTG-3’
	3-CTTGGTTCGAAGTCTCGGATCGGT-5’	323
CCK-BR	5-GTGGCCTACGGGCTTATCTCTCGCGAGCTCTACTTA-3’
	3-GACACAACCAACGGTCAAATATCACGGTTGTGCA-5’	357
β-actin	5-GGCGGCACCACCATGTACCCT-3’
	3-TCATACTGCTCAGGCCGGGGA-5’	202

Table 2 mRNA expression of IGF-1/IGF-1R and gastrin/CCK-BR in gastric cancer tissues and normal mucosa from controls (mean±SE).

Type of specimen	samples	IGF-1/β-actin	IGF-1R/β-actin	Gastrin/β-actin	CCK-BR/β-actin
Cancer tissues	56	0.305±0.032b	0.129±0.011b	0.293±0.031b	0.159±0.018b
Controls	56	0.154±0.013	0.027±0.004	0.169±0.017	0.058±0.006

^bP<0.01 vs normal mucosa from controls.

Table 3 mRNA expression of IGF-1/IGF-1R and gastrin/CCK-BR in three kinds of tissues from 56 patients with gastric carcinoma (mean±SE).

Test factor/β-actin	Cancer tissue	Adjacent mucosa	Tumor free
IGF-1/β-actin	0.305±0.028c	0.220±0.023a	0.149±0.012
IGF-1R/β-actin	0.129±0.011c	0.089±0.007a	0.047±0.006
Gastrin/β-actin	0.284±0.024c	0.182±0.014	0.133±0.043
CCK-BR/β-actin	0.169±0.016a	0.144±0.013a	0.049±0.014

^aP<0.05 vs tumor-free tissues.

^cP<0.05 vs adjacent mucosa.