Effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cells

Table 1 Influence of different concentrations of NCTD on Matrigel invasion of GBC-SD cells.

Group	NCTD (mg/mL)	n	Passing-membrane cells (% of control group)	Dead passing-membrane cells (% of control group)
Control	0	6	100.0	17.2±1.15
NCTD	5	6	62.0±5.08b	31.7±4.29b
	10	6	37.7±2.01b	59.7±1.15b
	20	6	18.0±1.79b	67.3±4.93b
	40	6	5.0±1.80b	95.2±4.88b

^bP<0.01 vs control group.

Table 2 Influence of different concentration of NCTD on the crossing-river time of GBC-SD cells.

Group	NCTD (mg/mL)	n	Crossing-rivertime (h)
Control	0	6	22.6±0.67
NCTD	5	6	28.2±1.00b
	10	6	33.4±0.71b
	20	6	45.0±0.68b
	30	6	58.8±0.72b
	40	6	>72b

^bP<0.01 vs control group.

Table 3 Influence of NCTD on expression of PCNA, Ki-67 proteins of GBC-SD cells.

Group	n	Positive index (mean±SD)
		PCNA	Ki-67
Control	10	0.932±0.031	0.964±0.092
IC50 NCTD (56.18 mg/mL)	10	0.318±0.023a	0.297±0.018a

^aP<0.05 vs control group.

Table 4 Influence of NCTD on expression of MMP₂, TIMP₂ proteins and the MMP₂ to TIMP₂ ratio of GBC-SD cells.

Group	NCTD (mg/mL)	n	Percent of protein expression (%)		MMP2/TIMP2
			MMP2	TIMP2
Control	0	10	62.82±3.20	13.42±1.27	4.68
NCTD	5	10	34.61±1.82a	30.79±2.15a	1.12b
	10	10	27.02±1.40a	34.22±2.97a	0.79b
	20	10	26.18±1.81a	35.60±2.04a	0.74b
	40	10	19.56±2.36 a	29.54±2.33a	0.66b

^aP<0.05 vs control group;

^bP<0.01 vs control group.