Changes of ECM and CAM gene expression profile in the cirrhotic liver after HCV infection: Analysis by cDNA expression array

Table 1 Laboratory and clinical summary data of all patients.

	Sex (F/M)	Age (yr)	TBIL (mmol/L)	ALB (g/L)	Encephalopathy	Ascites	Nutritional state	Child class	Gross shape1
1	M	40	17.7	36.0	No	No	Fine	A	Great
2	F	45	17.4	36.9	No	No	Fine	A	Great
3	F	49	19.8	41.6	No	Small	Fine	B	Great
4	M	41	52.3	31.2	No	Mess	Weak	C	Diffuse
5	M	37	39.1	40.6	No	Small	Fine	B	Great
6	F	41	26.1	43.9	No	No	Fine	A	Less
7	M	47	10.2	40.1	No	No	Fine	A	Diffuse
8	M	55	22.5	33.8	No	Small	Fine	B	Less
9	F	38	19.6	28.4	No	Small	Fine	C	Less
10	F	40	7.6	45.3	No	Small	Fine	A	Diffuse
11	M	59	70.3	35	No	Small	Fine	C	Great
12	M	48	46.5	45.4	No	Small	Weak	B	Great

Note:

¹great – greater tubercle type of cirrhosis; less – lesser tubercle type of cirrhosis; diffuse – diffuse type of cirrhosis.

Table 2 Health assessment survey and screen.

Patient		Sex	Age (yr)	Progress (yr)	Child score	Liver screen and appearance
Test group	1	F	45	11	A	Greater tubercle liver cirrhosis portal vein: 1.1±0.1 cm in diameter
	2	F	49	10	B
	3	M	40	4	A
Control group	1	F	35	-	A	Normal
	2	F	46	-	A
	3	M	31	-	A

Table 3 Up-regulated genes in liver cirrhosis (Exp.1 = normal, Exp.2 = liver cirrhosis, N/A = 0.00 in normal).

Gene name	Exp.1	Exp.2	Exp.2-Exp.1	Exp.2/Exp.1
CAM
ITGAa10	3.531E-4	5.751E-3	5.398E-3	1.63E+01
ITGA 7	-0.000E-1	1.722E-5	1.722E-5	N/A
ITGA L	2.639E-3	7.921E-3	5.282E-3	3.00E+00
ITGA M	6.690E-4	7.043E-3	6.374E-3	1.05E+01
ITGA V	2.416E-4	2.635E-3	2.393E-3	1.09E+01
ITGA X	-0.000E-1	2.497E-3	2.497E-3	N/A
ITGAb2	-0.000E-1	1.877E-3	1.877E-3	N/A
ICAM1	4.296E-2	3.695E-1	3.265E-1	8.60E+00
CD44	3.847E-3	9.706E-2	9.322E-2	2.52E+01
CTNN1	1.728E-1	5.052E-1	3.324E-1	2.92E+00
CST3	1.057E-1	3.735E-1	2.678E-1	3.53E+00
ECM
CAV1	5.017E-4	5.699E-3	5.198E-3	1.14E+01
COL1A1	5.947E-4	7.266E-3	6.672E-3	1.22E+01
LAMb1	1.301E-4	8.969E-2	8.956E-2	6.90E+02
LAMC1	-0.000E-1	3.444E-5	3.444E-5	N/A
SPP1	2.973E-4	7.781E-1	7.778E-1	2.62E+03
THBS1	1.639E-2	7.918E-1	7.754E-1	4.83E+01
Metalloproteinases
ADAMTS1	-0.000E-1	1.860E-1	1.860E-1	N/A
ADAMTS 8	7.433E-5	7.404E-4	6.661E-4	9.96E+00
MMP15	1.858E-5	6.888E-5	5.029E-5	3.71E+00
MMP 16	-0.000E-1	3.788E-4	3.788E-4	N/A
MMP 17	-0.000E-1	6.888E-5	6.888E-5	N/A
MMP 2	3.717E-5	2.411E-4	2.039E-4	6.49E+00
MMP 8	-0.000E-1	4.305E-4	4.305E-4	N/A

Table 4 Down-regulated genes in liver cirrhosis (Exp.1 = normal, Exp.2 = liver cirrhosis).

Gene name	Exp.1	Exp.2	Exp.2-Exp.1	Exp.2/Exp.1
CAM
Integrin
ITGA3	3.529E-1	9.885E-2	-2.541E-1	2.80E-01
ITGA5	1.388E-2	4.546E-3	-9.336E-3	3.28E-01
ITGA8	1.067E-2	2.927E-4	-1.037E-2	2.74E-02
Cadherin	2.604E-1	5.863E-2	-2.018E-1	2.25E-01
CTNND2
ECM protein:	6.948E-2	3.960E-3	-6.552E-2	5.70E-02
SPARC
Serine proteinases
CTSG	1.468E-2	8.954E-4	-1.379E-2	6.10E-02
TMPRSS4	1.191E-2	-0.000E-1	-1.191E-2	0.00E+00
HPSE	2.531E-1	3.306E-2	-2.201E-1	1.31E-01
Tissue inhibitor of metalloproteinases
TIMP2	2.895E-2	-0.000E-1	-2.895E-2	0.00E+00
TIMP 3	6.168E-2	3.960E-4	-6.128E-2	6.42E-03